High Coverage Gene Expression Profiling - HiCEP

Enlarge Image

Figure 1:HiCEP workflow, click to enlarge.

The HiCEP method is an amplified fragment length polymorphism (AFLP)-based gene expression profiling method that requires no prior sequence information and has a reduced rate of false positives and a high degree of detection of both coding and non-coding transcripts.

Whole transcriptome expression profiling is historically performed using hybridization-based microarray methods. There are a number of limitations to microarray-based approaches such as low sensitivity and specificity, poor dynamic range, as well as a need for prior sequence information. The HiCEP method was developed to address the shortcomings in gene expression profiling and provide a sensitive method for detecting a large proportion of transcripts in both known and unknown genes, with a low false positive rate.