Amplified Fragment Length Polymorphism-AFLP® Analysis
AFLP® is a technique used to detect polymorphisms in DNA when no information about the genome is known. Following restriction enzyme digestion of DNA, a subset of DNA fragments is selected for PCR amplification and visualization. A unique fingerprint is generated for a particular genome. AFLP®, first developed for plant studies, is now used for a wide variety of genetic analysis applications.
The power of AFLP® analysis is the speed with which the technique can quickly generate large numbers of marker fragments for any organism, without the need for any sequence data.
Step-by-Step Guide to AFLP® Analysis
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.
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