AFLP®, RFLP, SSCP, and BAC Fingerprinting
 | AFLP®, RFLP, SSCP, and BAC fingerprinting are all widely used and proven techniques for genetic mapping and/or linkage analysis. |
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Amplified Fragment Length Polymorphism (AFLP®)
 Amplified Fragment Length Polymorphism (AFLP®)
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Single-Strand Conformation Polymorphism (SSCP)/Heteroduplex Mobility Assay
 DNA that contains a sequence mutation (even a single base pair change) has a measurable mobility difference compared to wild type DNA when subjected to nondenaturing (or partially denaturing) capillary electrophoresis conditions. This mobility difference is the basis of SSCP/heteroduplex analysis. |
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Restriction Fragment Length Polymorphisms (RFLPs/tRFLPs)
 RFLPs are generated by digesting DNA with restriction enzymes. The resulting DNA fragments are then separated by size using capillary electrophoresis. A mutation that coincidentally affects a restriction site will appear as a fragment-length aberration compared to fragment pattern given by wild type DNA. |
Bacterial Artificial Chromosome (BAC) Fingerprinting
 BAC fingerprinting is a technique used for building physical maps of chromosomes by assembling large-insert (genomic) libraries such as those generated by restriction enzyme digestion. |
