AFLP®, RFLP, SSCP, & BAC Fingerprinting

AFLP®, RFLP, SSCP, and BAC Fingerprinting

AFLP®, RFLP, SSCP, and BAC fingerprinting are all widely used and proven techniques for genetic mapping and/or linkage analysis.


Amplified Fragment Length Polymorphism (AFLP®) Amplified Fragment Length Polymorphism (AFLP®) AFLP® is a technique used to detect polymorphisms in DNA when no information about the genome is known. Learn more		Single-Strand Conformation Polymorphism (SSCP)/Heteroduplex Mobility Assay DNA that contains a sequence mutation (even a single base pair change) has a measurable mobility difference compared to wild type DNA when subjected to nondenaturing (or partially denaturing) capillary electrophoresis conditions. This mobility difference is the basis of SSCP/heteroduplex analysis. Learn more


Restriction Fragment Length Polymorphisms (RFLPs/tRFLPs) RFLPs are generated by digesting DNA with restriction enzymes. The resulting DNA fragments are then separated by size using capillary electrophoresis. A mutation that coincidentally affects a restriction site will appear as a fragment-length aberration compared to fragment pattern given by wild type DNA. Learn more		Bacterial Artificial Chromosome (BAC) Fingerprinting BAC fingerprinting is a technique used for building physical maps of chromosomes by assembling large-insert (genomic) libraries such as those generated by restriction enzyme digestion. learn more