No More Western Myths
MYTH: Dry blotting does not produce an efficient transfer of protein for detection.
FACT: Dry blotting is just as efficient at protein transfer as wet blotting.
|The iBlot® 7-Minute Blot is built upon an optimized interaction of consumables chemistry and an electrical field, which typically results in a transfer 10 times faster than traditional wet or semi-wet blotting. Research around this technology has been conducted to improve or alter protein transfer patterns and efficiency using simple methods. Data demonstrates that overall transfer to nitrocellulose membranes can be optimized by using:|
These two simple changes enable improvements in the transfer of large proteins while maintaining smaller proteins. Both changes provide iBlot® system users with high-quality, quantitative, and reproducible results.
Novex® Tris-Glycine 4–20% transferred to an iBlot® PVDF membrane and detected using a WesternBreeze™ kit. Lanes 1–5: E. coli lysate, detected with an anti–E. coli antibody, (1.25 μg in lane 1, serial 2-fold dilution in lanes 2–5). (A) iBlot® 7-Minute Blot transfer using program P3 for 7 minutes. (B) iBlot® 7-Minute Blot transfer using program P3 for 7 minutes, following 10 minutes of gel equilibration in 20% ethanol. Red rectangles indicate areas of elevated detection sensitivity for both large and small molecular weight proteins.
MYTH: Using the iBlot® 7-Minute Blot to transfer is more expensive than wet blotting.
FACT: Using the iBlot® 7-Minute Blot is very cost efficient when comparing to traditional wet transfer methods.
Traditional wet transfer hasn’t changed much over the last 30 years. Using the iBlot® device for your protein transfers can actually save you money.
Meet the Inventor: iBlot® System
What does it really cost to prepare and perform a traditional wet transfer and an iBlot® 7-Minute Blot transfer?
- View the Protein Transfer Comparison Chart.
Still want to do wet transfer?
Make it fast, simple, and easy with Novex® ready-to-use blot modules!