Western Blot Transfer Myths

No More Western Myths
Yes Novex®!

It's about time old western transfer myths were busted. Bring your protein transfer tools up to date today with Novex® western transfer equipment.

Western Blot Transfer Myths:

1. Dry blotting does not produce an efficient transfer of protein for detection

2. Using the iBlot® 7-Minute Blot to transfer is more expensive than wet blotting

 

Learn more about Novex® western transfer products

MYTH: Dry blotting does not produce an efficient transfer of protein for detection.
FACT: Dry blotting is just as efficient at protein transfer as wet blotting.


The iBlot® 7-Minute Blot is built upon an optimized interaction of consumables chemistry and an electrical field, which typically results in a transfer 10 times faster than traditional wet or semi-wet blotting. Research around this technology has been conducted to improve or alter protein transfer patterns and efficiency using simple methods. Data demonstrates that overall transfer to nitrocellulose membranes can be optimized by using:
  • A 3-step voltage program instead of a single-step constant voltage
  • Ethanol in water for pretransfer equilibration (instead of using a gel transfer buffer)

These two simple changes enable improvements in the transfer of large proteins while maintaining smaller proteins. Both changes provide iBlot® system users with high-quality, quantitative, and reproducible results.

 
iBlot® System in Action

See the iBlot® System in Action

Western Blot transfer

  

 

Novex® Tris-Glycine 4–20% transferred to an iBlot® PVDF membrane and detected using a WesternBreeze™ kit. Lanes 1–5: E. coli lysate, detected with an anti–E. coli antibody, (1.25 μg in lane 1, serial 2-fold dilution in lanes 2–5). (A) iBlot® 7-Minute Blot transfer using program P3 for 7 minutes. (B) iBlot® 7-Minute Blot transfer using program P3 for 7 minutes, following 10 minutes of gel equilibration in 20% ethanol. Red rectangles indicate areas of elevated detection sensitivity for both large and small molecular weight proteins.

 Learn more about the iBlot® 7-minute Blotting System

MYTH: Using the iBlot® 7-Minute Blot to transfer is more expensive than wet blotting.
FACT: Using the iBlot® 7-Minute Blot is very cost efficient when comparing to traditional wet transfer methods.

 

Traditional wet transfer hasn’t changed much over the last 30 years. Using the iBlot® device for your protein transfers can actually save you money.

  • Less preparation time—you can get your results faster, without the hassle of preparing so many buffers
  • Better blot-to-blot consistency—your transfer is more consistent, without the additional variables that multiple buffers add to the transfer
 
Meet the Inventor: iBlot® System

Meet the Inventor: iBlot® System

Cost Comparison

What does it really cost to prepare and perform a traditional wet transfer and an iBlot® 7-Minute Blot transfer?


Learn more about the iBlot® 7-minute Blotting System

 

Western Transfer Showdown:
Wet Blot vs. Dry Blot

Did you know you can perform a western protein transfer without buffer in 7 minutes? Watch this playful look at the Western Protein transfer process.

Still want to do wet transfer?

Make it fast, simple, and easy with Novex® ready-to-use blot modules!

Resources

Application Notes
FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAPEUTIC OR DIAGNOSTIC USE.
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