iBlot® 7-Minute Blotting System

  • Fast— protein transfer in 7 minutes or less
  • High quality transfer— efficient transfers without bubbles or distortions
  • Convenient—Self contained unit without the need for additional buffer or external power supply
  • Reproducible—reduces blot preparation and running variability

Detection Kits For Results in 30 Minutes

Compare To Semi-Dry & Wet Transfer Systems

iBlot® System Customer Ratings & Reviews

Self-Contained Unit for Faster, More Convenient Transfers

The iBlot® 7-Minute Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. A self-contained unit, the iBlot® device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes (Figure 1), offering the convenience of a bufferless, plug-and-play system. 

iBlot Dry Blotting System  
Figure 1.  How iBlot® dry blotting works.  Instead of layered filter paper or buffer tanks, the top and bottom stacks contain the necessary buffers.  The bottom stack includes an integrated 0.2 µm nitrocellulose or PVDF membrane

High Transfer Efficiency

In addition to ease and convenience, the iBlot® system offers high-efficiency protein transfers (Figure 2) for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, you’ll achieve more accurate detection using less sample. 

Figure 2. High transfer efficiencies achieved using the iBlot® 7-Minute Blotting System.  A. iBlot® dry transfer to nitrocellulose and B. semi-dry transfer to nitrocellulose of NuPAGE® Novex® 12% Bis-Tris Mini Gels.  Lanes 1-6: 0.0625 µg, 0.25 µg, 0.5 µg. 1.0 µg, and 2.0 µg SW480 human colon cancer cell lysate; lanes 7 and 12; 5 µL SeeBlue® Plus2 Pre-Stained Protein Standard; lanes 8-11: 0.5 µL, 1.0 µL, 2.0 µL, 4.0 µL MagicMark™ XP Western Protein Standard.

Fits your gel size

The iBlot® 7-Minute Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size, fitting Novex® Midi Gels (8 x 13 cm), E-PAGE™ 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm).

Already have an iBlot® Gel Transfer Device? 

Registering your iBlot® Gel Transfer Device allows us to keep you informed of future firmware upgrades. 

Register your iBlot® Gel Transfer Device here

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