Western Blot Transfer Systems

One of the key steps in  the Western blot workflow is the transfer of proteins from the polyacrylamide gel after electrophoresis to the nitrocellulose or polyvinylidene difluoride (PVDF) membrane so that specific proteins can be detected using immunodetection techniques.

Methods for achieving this protein transfer include capillary transfer or electrophoretic transfer. Life Technologies has electrophoretic transfer systems using wet, semi-dry and dry methods outlined below.

Which Western Transfer System is Right for You?

  Traditional wet transfer in 1-2 hours Semi-dry transfer for high efficiency Dry Transfer in 7 minutes
Transfer time 60-120 minutes 30-60 minutes 7 minutes
Capacity of device 1-2 mini gels 4 mini gels or
2 midi gels		 2 mini gels or
1 midi gel
Blotting Area 9 x 9 cm 21 x 21 cm 13.5 x 8.5 cm
Requires Transfer buffer Yes (1000 ml) Yes (500 ml) No
Power supply External External Internal
US Device List Price $354.77 $1,089.42 $1,549.71
Estimated price per mini gel transfer $15.00 $9.00 $7.00
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Transfer Buffers

 

  • Convenient pre-mixed buffers ensure high quality, consistent results

  • Made with high-quality reagents and strict quality control

  • Available in concentrated form for dilution prior to use

See Pre-Mixed Buffers for Protein Electrophoresis

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