Polyvinylidene difluoride (PVDF) membrane is ideal for western blotting applications as well as for amino acid analysis and protein sequencing of small amounts of proteins (as little as 10 pmoles). In addition, PVDF membranes can be used stripped and reprobed without a loss of sensitivity or increased background.
Applications & Specifications for PVDF Membranes
|Specifications||Invitrolon™ PVDF||0.2 µm PVDF|
|Cat no.||LC2005 (20 transfers)||LC2002 (20 transfers)|
|Quantity||20 membrane/filter paper sandwiches||20 membrane/filter paper sandwiches|
|Pore Size||0.45 µm||0.2 µm|
|Dimensions||8.3 cm x 7.3 cm||8.3 cm x 7.3 cm|
|Binding Capacity||Goat IgG: 294 µg/cm2BSA: 131 µg/cm2Insulin: 85 µg/cm2||50-150 µg/cm2 for large, globular proteins>150 µg/cm2 for smaller peptides|