Western Blot Membranes
Life Technologies makes blotting easier and more convenient by providing a variety of Novex® pre-cut, pre-assembled western blot membrane/filter-paper sandwiches that perfectly fit mini-gels, E-PAGE™ gels, and comparable size gels. PVDF (polyvinylidene difluoride) and nitrocellulose, membranes are available in different pore sizes and dimensions for different application needs. A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces so finding the optimal membrane may require experimenting with your specific protein on different membranes. Below are some basic guidelines to help you choose from Invitrogen™ western blot membranes available from Life Technologies. |
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Choosing Your Western Blot Membrane
| Membrane Type | Applications & Uses* | Re-probe Characteristics | Binding Interaction |
|---|---|---|---|
| Nitrocellulose Membranes | WB-low mol wt proteins (<20 kDa), S,N-nucleic acids (<300 bp), AA, DSB | Cannot Be Striped & Reprobed | Hydrophobic and Electrostatic |
| iBlot® Transfer Stack, Nitrocellulose Membrane | WB-low mol wt proteins (<20 kDa) | Cannot Be Striped & Reprobed | Hydrophobic and Electrostatic |
| PVDF Membrane | WB, P, AA, SPA | Can Be Striped & Reprobed | Hydrophobic |
| Invitrolon™ PVDF Membrane | WB- proteins > 10 kDa, P, AA | Can Be Striped & Reprobed | Hydrophobic |
| iBlot® Transfer Stack, PVDF Membrane | WB | Can Be Striped & Reprobed | Hydrophobic |
| *WB=Western Blot Transfer, S=Southern Transfer, N=Northern Transfer, AA=Amino Acid Analysis, DBS=Dot/Slot Blotting, P=Protein Sequencing, SPA=Solid Phase Assay Systems. | |||
