Western Blot Detection

Invitrogen offers a broad range of reagents and kits for western blot detection. Whether you are using chromogenic (colorimetric), fluorescent, or chemiluminescence detection systems, we have the solutions you need.

Quickly find all of our Primary Antibodies Validated for Western Blot Analysis

iBlot® Western Immunodetection Kits

iBlot® Immunodetection Kits      

The iBlot® Western Immunodetection Kits offer comparable or better sensitivity than the conventional protocols for the majority of the antibody-antigen pairs while providing significant time savings. They enable complete western detection in less than 25 minutes, rather than 3–5 hours.

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Novex® WesternBreeze™ Immunodetection Kits

WesternBreeze™ Immunodetection Kits      

The Novex® WesternBreeze™ Immunodetection Kits provide all the solutions you need for a western blot, all in one kit. Solutions are pre-mixed and optimized to provide reproducible blots, time after time, using just one fast, simple protocol. Develop with your choice of either a chromogenic or chemiluminescent substrate.

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WesternDot™ Western Blot Immunodetection Kits

WesternDot™ Western Blot Immunodetection Kits      

The WesternDot™ Western Blot Immunodetection Kits offer the unique properties of our fluorescent Qdot® 625 nanocrystals combined with the high-affinity streptavidin-biotin binding reaction to give you straightforward, high-sensitivity detection of proteins on western blots. Get results equivalent to routine ECL and stop multiple trips to the darkroom to process film.

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Novex® ECL Chemiluminescent Substrate Reagent Kit

Novex® ECL Chemiluminescent Substrate Reagent Kit      

The Novex® ECL Chemiluminescent Substrate Reagent Kit provides detection sensitivity superior to that of precipitating chromogenic substrates. Using a horseradish peroxidase, low picogram levels of detection can be achieved using either X-ray film or imaging equipment. Light emission for Novex® ECL Chemiluminescent Substrate is most intense from 5–30 minutes after membrane incubation and decreases slowly with time over the course of several hours.

Table 1 - Western Blot Detection Kits

 ProductSensitivityEmission DurationAdditional Equipment RequiredCat. No.
WesternBreeze® Chromogenic KitsLow PicogramMonths to Years
  • None
  • Optional:  white light camera or scanner 
     
WesternBreeze® Chemiluminescent KitsHigh FemtogramDays
  • Darkroom
  • autorad/ x-ray film & developer
  • Scanner
  • Luminescent imager
     
Novex® ECL Chemiluminescent KitHigh FemtogramHours
  • autorad/ x-ray film & developer
  • Scanner
  • Luminescent imager
     
WesternDot™ Fluorescent Detection KitsLow PicogramDays to Months
  • UV-transiluminator or UV-Illuminator
  • Digital camera with orange/red filter
  • Laser scanner