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iBlot® Dry Blotting System

  • Fast—complete protein transfer in 7 minutes or less
  • Reproducible—reduces blot preparation and running variability
  • High-quality transfer—straight images without bubbles or distortions
  • Convenient self-contained unit—no added buffers or external power supply needed

Self-contained unit for faster, more convenient transfers

  • The iBlot® Dry Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. A self-contained unit, the iBlot® device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes (Figure 1), offering the convenience of a bufferless, plug-and-play system.





         		  
    Figure 1.  How iBlot® dry blotting works.      Instead of layered filter paper or buffer tanks, the top and bottom stacks contain the necessary buffers.  The bottom stack includes an integrated 0.2 µm nitrocellulose or PVDF membrane

High Transfer Efficiency

  • In addition to ease and convenience, the iBlot® system offers high-efficiency protein transfers (Figure 2) for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, you’ll achieve more accurate detection using less sample.

        Figure 2. High transfer efficiencies achieved using the iBlot® Dry Blotting System.  A. iBlot® dry transfer to nitrocellulose and B. semi-dry transfer to nitrocellulose of NuPAGE® Novex® 12% Bis-Tris Mini Gels.  Lanes 1-6: 0.0625 µg, 0.25 µg, 0.5 µg. 1.0 µg, and 2.0 µg SW480 human colon cancer cell lysate; lanes 7 and 12; 5 µl SeeBlue® Plus2 Pre-Stained Protein Standard; lanes 8-11: 0.5 µl, 1.0 µl, 2.0 µl, 4.0 µl MagicMark™ XP Western Protein Standard.

Fits your gel size

  • The iBlot® Dry Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size, fitting Novex® Midi Gels (8 x 13 cm), E-PAGE™ 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm).