Lipofectamine® RNAiMAX Transfection Reagent - Delivery of siRNA
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Get transfection perfection in your gene knockdown studies
Lipofectamine® RNAiMAX Transfection Reagent offers an advanced, efficient solution for siRNA delivery. No other siRNAspecific transfection reagent provides such easy and efficient siRNA delivery in a wide variety of cell lines including common cell types, stem cells and primary cells as well as traditionally hard-to-transfect cell types.
siRNA Transfection Protocol
 | How to perform siRNA transfection with Lipofectamine® RNAiMAX protocol. Superior siRNA/miRNA delivery and gene knockdown |
Superior transfection at low siRNA concentrations
When it comes to achieving effective gene knockdown, Lipofectamine® RNAiMAX Transfection Reagent easily outperforms other siRNA transfection reagents. High knockdown levels of target genes can be achieved with as little as 1 nM siRNA (Figure 1). The Lipofectamine® RNAiMAX protocol recommends using 10 nM siRNA as a starting point to maximize knockdown levels.
Figure 1: Superior knockdown with Lipofectamine® RNAiMAX Transfection Reagent compared to competing siRNA transfection reagents. At both 10 nM and 1 nM p53 siRNA, Lipofectamine® RNAiMAX Transfection Reagent provides more effective knockdown relative to nontransfected cells than other RNAi reagents, including siLentFect™ (Bio-Rad), DharmaFECT™ (Dharmacon), and HiPerFect (Qiagen) reagents.
Low cytotoxicity profile for easy optimization
Lipofectamine® RNAiMAX Transfection Reagent gives maximal knockdown and excellent cell viability across a 10-fold concentration range of the reagent (Figure 2). This makes Lipofectamine® RNAiMAX reagent easy to optimize for the lowest siRNA concentration while reducing cytotoxicity in your experimental system. Transfection-mediated cytotoxicity can mask the true phenotype of a target gene being studied, so minimizing the amount of reagent used in your transfections is a critical factor for successful RNAi experiments.
Figure 2: Optimal knockdown and minimal cytotoxicity in A549 cells transfected with Lipofectamine® RNAiMAX Transfection Reagent. A range of 0.1 μl to 1.0 μl of Lipofectamine® RNAiMAX reagent was used, resulting in efficient knockdown of the p53 gene expression level while maintaining excellent cell viability.
Optimized protocols available for many cell lines
With its ability to transfect a broad range of cell types, Lipofectamine® RNAiMAX reagent, combined with the BLOCK-iT™ Alexa Fluor® Red Fluorescent Control, gives you the most versatile approach to all of your knockdown experiments (Table 1, Figure 3). Lipofectamine® RNAiMAX offers many streamlined cell line–specific protocols—no need to remove transfection complexes, or change or add medium following transfection. You’ll spend less hands-on time and get results faster.
Table 1: Achieve transfection perfection on a wide range of cell types with Lipofectamine® RNAiMAX Transfection Reagent.
| Cell line/type | Cell type |
|---|---|
| MDA-MB-435 | Breast cancer |
| HeLa | Cervical carcinoma |
| HT1080 | Human fibrosarcoma |
| A549 | Lung carcinoma |
| HepG2 | Liver carcinoma |
| MCF7 | Breast cancer |
| SK-N-SH | Neuroblastoma |
| Mesenchymal stem cells | Bone marrow |
| HAMSC | Human aortic smooth muscle cells |
| HEKa | Human Epidermal Keratinocytes |
| HRE | Human renal epithelial cells |
| NTERA-2 | Cells-embryonal carcinoma |
| HUVEC | Human umbilical vein endothelial cells |
| ADSC | Adipose-derived stem cells |
| HCT116 | Colon carcinoma |
Figure 3: Improve your transfection efficiencies using the BLOCK-iT™ Alexa Fluor® Red Fluorescent Control. HeLa cells were transfected with the BLOCK-iT™ Alexa Fluor® Red Fluorescent Control (50 nM) using Lipofectamine® RNAiMAX Transfection Reagent. A. Twenty-four hours after transfection, nearly 100% of the cells take up the control and retain a normal morphology as seen in the bright-field image. B.
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