Surface-Activated Dynabeads®
Dynabeads® with specific active groups on their surface allow flexible experimental design in a wide range of proteomic , molecular and cellular applications, including affinity isolations and bioassays. Each product offers specific surface chemical functionalities, allowing convenient binding of a wide range of ligands for maximum flexibility for target isolation.
Product Advantages:
See our new product Co-IP Kit based on Surface Activated Dynabeads M-270 Epoxy
Product Advantages:
- Gentle, rapid and simple single-tube manipulations
- Convenient and versatile technology, offering flexible experimental design
- Efficient protocol-no columns or centrifugation steps eliminate losses
- Low non-specific binding of proteins and absolute reproducibility with monosized magnetic beads
See our new product Co-IP Kit based on Surface Activated Dynabeads M-270 Epoxy
Figure 1. Antibodies or other ligands can be coupled onto the beads for affinity protein capture.
A Wide Range of Products - Flexibility for Your Research
Surface-activated Dynabeads® are available in different sizes, with a hydrophobic or hydrophilic nature, and with a variety of surface chemistries (see Table 1 below). To obtain the best possible results it is important to choose a size and surface of the Dynabeads® suited for your application. The choice will depend on the nature of the ligand. In most applications several Dynabeads® products can be used.
Table 1 - Choose the right surface-activated Dynabeads® for your application
| Ligand | Target | Dynabeads® Tosyl- activated | Dynabeads® Carboxylic Acid | Dynabeads® Amine | Dynabeads® Epoxy | Dynabeads® Subcellular |
|---|---|---|---|---|---|---|
| Antibody | Low MW antigen1 or peptide |  | | | | |
| Protein or antibody | | | | | ||
| Protein complex | | | | | ||
| Organelles | | 2 | | |||
| Phage1 | | | | | ||
| Virus | | | | | ||
| Bacteria | | | ||||
| Cells3 | | | | |||
| Antibody Fragment | Phage1 or antibody | | | | | |
| Protein | Phage or carbohydrate | | | | | |
| Nucleic acid | | | | | ||
| Peptide | Phage1 or antibody | | | | | |
| Carbohydrate | Antibody | | | | ||
| Low MV antigen | Antibody | | | | | |
| Nucleic acids, oligonucleotide, aptamer, PNA | Nucleic acid binding proteins1 | | | 4 | ||
| DNA1, RNA1 or PCR amplicons1 | | 4 | ||||
| Enyme | Substrate or target for enzyme degradation | | | | | |
| Organic chemistry derivatization, including introduction of new functional groups | | | | |
- Best product choice for listed ligand and target. - Alternative choice for listed ligand and target. - Can be used for listed ligand and target.1 - Dynabeads® pre-coupled with streptavidin may be the best product choice.
2 - Dynabeads® M-450 Epoxy.
3 - Dynabeads® M-450 are recommended for isolation, stimulation, and expansion of cells.
4 - Dynabeads® Amine is modified with an appropriate bifunctional crosslinker prior to immobilization of oligonucleotides.
