Protein Isolation Product Guide
This guide gives information on Dynabeads® that can be coated with different ligands. Dynabeads are available with many different surfaces, each having unique affinities tailored for different ligands and assays. Most of the beads listed in the table bind their ligand with a non-covalent interaction.
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| Dynabeads Product | Bead Characteristics | Binding Properties | Type of ligand | Coupling |
|---|---|---|---|---|
| Protein A (for IP) | Size: 2.8 µm Surface: protein A | non-covalent antibody binding | many antibodies: see guide | >10 min. room temp. |
| Protein G (for IP) | Size: 2.8 µm Surface: protein G | non-covalent antibody binding | many antibodies: see guide | 10–40 min. room temp. |
Co-IP Kit | Size: 2.8 µm Surface: Epoxy (ultra low background) | covalent antibody binding *antibody will not elute off beads | Any antibody | 15 min. setup (overnight reaction) |
| His-Tag Isolation & Pulldown | Size: 1 µm Surface: Cobalt NTA Chemistry | ionic interaction very low background | Binds Histidine tagged (His-Tagged) proteins | 5 min. room temp. |
Antibody Coupling Kit | Size: 2.8 µm Surface: Epoxy (ultra low background) | covalent binding of antibodies and other desired proteins | Binds any antibody & any protein ligand including functional enzymes | 15 min. setup (overnight reaction) |
M-280 Sheep α-Mouse IgG | Size: 2.8 µm Surface: affininty purified polyclonal sheep α-mouse IgG antibodies (covalently bound) | non-covalent antibody binding | Binds Mouse IgG1, IgG2a, IgG2b | > 30 min. 2 – 8°C |
M-280 Sheep α-rabbit IgG | Size: 2.8 µm Surface: affinity purified polyclonal sheep α-rabbit IgG antibodies (covalently bound) | non-covalent antibody binding | Binds any Rabbit IgG antibody | > 120 min. 2 – 8°C |
M-280 Streptavidin | Size: 2.8 µm Surface: hydrophobic beads with covalently bound streptavidin | Binds ~10 µg biotinylated antibodies per mg | Binds any biotinylated antibody, as well as other biotinylated ligands | 30 min. room temp. |
M-270 Streptavidin | Size: 2.8 µm Surface: hydrophilic beads with covalently bound streptavidin | Binds ~10 µg biotinylated antibodies per mg | Binds any biotinylated antibody, as well as other biotinylated ligands | 30 min. room temp. |
MyOne™ Streptavidin T1 | Size: 1 µm Surface: hydrophobic beads with covalently bound streptavidin | Binds ~20 µg biotinylated antibodies per mg | Binds any biotinylated antibody, as well as other biotinylated ligands | 30 min. room temp. |
MyOne™ Streptavidin C1 | Size: 1 µm Surface: hydrophilic beads with covalently bound streptavidin | Binds ~20 µg biotinylated antibodies per mg | Binds any biotinylated antibody, as well as other biotinylated ligands | 30 min. room temp. |
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