Dynabeads® coated with secondary antibodies
|Secondary-coated Dynabeads® (e.g. Dynabeads® M-280 Sheep anti-Rabbit IgG and Dynabeads® M-280 Sheep anti-Mouse IgG) are pre-coated with purified primary antibodies to allow maximum flexibility of your protein/peptide purification. A specific secondary antibody can also be added to isolate your specific target. |
For simple, robust, and rapid small-scale purification of immunoglobulins and subsequent isolation of target protein, (immunoprecipitation) you can also use Dynabeads® Protein A and Dynabeads® Protein G.
Figure 1. Dynabeads® binding kinetics are superior to traditional microtitre plates. The graph shows % binding of tumor necrosis factor to immobilised antibody as a function of time. (Courtesy of N-B Liabakk, Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Norway.)
Direct or Indirect Method
Both a direct and indirect purification method can be employed;