His-Tagged Protein Isolation & Pulldown using Dynabeads®

Dynabeads for isolating His-Tagged Proteins

Overview:
Beads are non-porous (solid surface):
  • Binding occurs on bead outer surface
  • Fast bead/target interaction (≤ 5 min.)
  • No upper size limit for target
  • Low background (~ 100% purity)
IMAC Surface Chemistry:
  • Cobalt Based
  • Completely Novel
  • Extremely low non-specific binding
  • Highly specific for Histidine Tags
Downstream options:
  • Elute purified His-tagged proteins
    •  or  
  • Leave proteins on Dynabeads for use in bait-prey pulldowns, phage display, etc.
Very Low Background Levels:
  • nearly 100% purity
his tag purification