His-Tagged Protein Isolation & Pulldown using Dynabeads®

Dynabeads for isolating His-Tagged Proteins

Overview:

  • Extremely low background binding
  • 20 minute His-Tag isolation protocol
  • ~30-60 min. Bait-Prey protocol
  • Adjust buffer stringency for your target

 

Beads are non-porous (solid surface):
  • Binding occurs on bead outer surface
  • Fast bead/target interaction (≤ 5 min.)
  • No upper size limit for target
  • Low background (~ 100% purity)
IMAC Surface Chemistry:
  • Cobalt Based
  • Completely Novel
  • Extremely low non-specific binding
  • Highly specific for Histidine Tags
Downstream options:
  • Elute purified His-tagged proteins
    •  or  
  • Leave proteins on Dynabeads for use in bait-prey pulldowns, phage display, etc.
Very Low Background Levels:
  • nearly 100% purity
his tag purification
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