IEF and Native Protein Markers
![]() | Novex® IEF and Native Protein Standards are specialty standards for applications that demand flexibility for native electrophoresis conditions and resolution at either high or low pI. Each standard has its own advantages. Please use the table and information below to help select the best protein standard for your application. Learn More About Novex® Protein Standards and Ladders |
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HELPFUL TIPProtein pI Estimation
The IEF Marker 3-10 is designed for use on horizontal and vertical Isoelectric Focusing (IEF) gels to determine the isoelectric point (pI) of unknown protein samples. |
Selection Guide — IEF and Native Markers
| Application | IEF Marker 3-10 | NativeMark™ Unstained |
|---|---|---|
| SDS-PAGE | NA | NA |
| Western Blot | NA | Good |
| Detection with UV Transillumination | NA | Good |
| Confirmation of Epitope-tagged Proteins | NA | NA |
| Immediate Band Identification | NA | NA |
| Sharp Bands | Good | Good |
| MW Estimation | NA | Best for Native Proteins |
| Monitor Migration during Electrophoresis | NA | NA |
| Silver Staining | Good | Good |
| MW/pI Range | pI 3.5–10.7 | 20–1230 kDa |
| IEF Gel | Best | NA |
| Cat. No. | 39212-01 | LC0725 |



