The E-PAGE™ High-Throughput (HTP) Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis. It consists of the E-PAGE™ precast gels, E-Base™ integrated devices, E-Holder™ platform, and free E-Editor™ 2.0 software.
Advantages of using the E-PAGE™ High-Throughput Precast Gel System include:
E-PAGE™ High-Throughput Precast Gel System Applications
- In-gel staining with Lumio™ Green Reagent (FIgure 1)
- Total protein staining (Coomassie®, silver, or fluorescent stains) (Figure 2)
- Western blotting (Figure 3)
Figure 1. In-gel staining with Lumio™ Green Reagent. Expressway™ HTP Mix was used to express various human kinase ORFs with the pEXP3-DEST Expressway™ vector. Reactions were electrophoresed on an E-PAGE™ 96 6% gel and analyzed using the Lumio™ Green Detection Kit.
Figure 2. Total protein staining using Commassie® R-250. Lanes M: 5 µl SeeBlue® Plus2 Pre-stained Std.; Lanes 2, 4, 21, 23: 10 µl MagicMark™ XP Std.; Lanes 6 & 19: 2 µl BenchMark™ His-tagged Std.; Lane 8: lysozyme 200 ng; Lane 10: Carbonic Anhydrase 200 ng; Lane 12: BSA 100 ng; Lanes 15 & 17: 5 µl of E. coli lysate. Other lanes: blank The gel stained with Commassie® R-250 following the recommended protocol in the E-PAGE™ 48 manual.
Figure 3. Western blotting with the E-PAGE™ Protein Electrophoresis System. 5 µl MagicMark™ XP Western Standard was loaded into each well of an E-PAGE™ 48 8% Gel and transferred to a pre-cut 0.2 µm nitrocellulose membrane (Cat. no. LC2009). The blot was probed with 1:1000 Anti-Xpress™ Antibody and detected using the WesternBreeze® Immunodetection Kit (anti-mouse) – Chemiluminescent (A) and Chromogenic (B) detection.