New Affordable Option for Protein Gel Electrophoresis

Each kit comes with a preselected SeeBlue® standard. Simply select the appropriate buffer for your run.

Get optimal separation of your proteins by choosing the right gel and running buffer combination. Here is brief summary of the available configurations. See the selection guide above for specific product ordering information.

Buffering systems

Novex® Tris-Glycine gels do not contain SDS and can be used to run proteins in native or denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.

The Tris-Glycine discontinuous buffer systems utilize three ions:

• Chloride (–) from the gel buffer serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Tris+ and Cl– (pH 8.65).
• Glycine (–) is the primary anion in the running buffer and serves as a trailing ion. Glycine is partially negatively charged and trails behind the highly charged chloride ions in the charged environment. The running buffer ions are Tris+, Gly–, and dodecylsulfate– (pH 8.3).
• Tris Base (+) is the common ion present in the gel buffer and running buffer. During electrophoresis, the gel and buffer ions in the Tris-Glycine system form an operating pH of 9.5 in the separation region of the gel.

Gel storage and shelf-life

For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase.