Novex® Tris-Glycine Gels
Novex® Tris-Glycine gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis with minor modifications for maximum performance in the pre-cast format. Unlike traditional Laemmli gels with a stacking gel pH of 6.8 and separating gel pH of 8.8, Novex® Tris-Glycine gels have a pH of 8.65 for both regions. Novex® Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.
Novex® Tris-Glycine gels are:
Choose the Right Novex® Tris-Glycine Gel
|Number of Wells||Gel Thickness||Gel Percentage||Gel Sizes|
Getting Started with Novex® Tris-Glycine Gels
Get optimal separation of your proteins by choosing the right combination of gel and running buffer. Here is brief summary of the available configurations. See “Choose the Right Novex® Tris-Glycine Gel” above to get the specific product ordering information. See “Choose The Right NuPAGE® Bis-Tris Gel” above to get the specific product ordering information. See Recommended Well Loading Volumes & Sample Loads.
To determine what type of gel should be used to separate your proteins, please refer to Figure 1 for the protein migration patterns. Gel Sizes Figure 1. Migration patterns of Invitrogen protein molecular weight standards on Novex® Tris-Glycine Gels. For optimal results, protein bands should migrate within the yellow shaded areas.
Novex® Tris-Glycine gels come in a variety of fixed concentrations from 4% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. Each box contains 10 gels.
Novex® Tris-Glycine gels are available in two different sizes: mini gels (8 cm x 8 cm) and midi gels (8 cm x 13 cm). Both mini gels and midi gels are available with 1.0 mm gel thickness but the 1.5 mm gel thickness is available only with the mini gel format. Novex® Tris-Glycine gels come in multiple-well formats, ranging from 1 well to 26 wells.
To determine what type of gel should be used to separate your proteins, please refer to Figure 1 for the protein migration patterns.
Figure 1. Migration patterns of Invitrogen protein molecular weight standards on Novex® Tris-Glycine Gels. For optimal results, protein bands should migrate within the yellow shaded areas.
Novex® Tris-Glycine gels do not contain SDS and can be used to run your proteins in native form or in denatured form. For denatured proteins, we recommend using Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.
The Tris-Glycine discontinuous buffer systems utilizes three ions:
- Chloride (–) from the gel buffer serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are Tris+ and Cl– (pH 8.65).
- Glycine (–) is the primary anion in the running buffer and serves as a trailing ion. Glycine is partially negatively charged and trails behind the highly charged chloride ions in the charged environment. The running buffer ions are Tris+, Gly–, and dodecylsulfate– (pH 8.3).
- Tris Base (+) is the common ion present in the gel buffer and running buffer. During electrophoresis, the gel and buffer ions in the Tris-Glycine system form an operating pH of 9.5 in the separation region of the gel.
Gel Storage and Shelf-Life
For best results, most Novex® Tris-Glycine gels should be stored at 4°C and used within 8 weeks of purchase. 16% and 18% Novex® Tris-Glycine gels should be used within 4 weeks of purchase.