Protein Gels

The first step in protein analysis is sample separation. Protein gel electrophoresis is the most common technique used to separate proteins from cell and tissue extracts, blood or other biological sources.  Traditionally, protein gels had to be poured just prior to the electrophoretic run and the steps involved in their creation could be time-consuming and could also introduce artifacts in the experiment because of large variations in the gel composition.

Figure 1. Migration patterns of Novex® Sharp Protein Standards (prestained and unstained) on NuPAGE® Bis-Tris Gels and NuPAGE® Tris-Acetate Gels. For optimal results, protein bands should migrate within the yellow shaded areas.  Migration patterns of protein standards on NuPAGE® Novex Gels

Which Protein Gel Is Right for You?

  Pour Your Own Essentials
Preassembled empty cassettes, buffers, reagents 		Pre-cast convenience with Laemmli chemistry Straight protein bands, longest shelf-life
Protein Resolution Medium Medium High
Protein Integrity Medium Medium High
Shelf Life NA 1 - 2 months 8 months (Tris-Acetate)
16 months (Bis-Tris)
Average Run Time 90 min 90 min 35 min
Separation Range 6 - 500 kDa 6 - 500 kDa 1.5 - 300 kDa (Bis-Tris)
30 - 400 kDa (Tris-Acetate)
Gel % Available NA 4%, 6%, 8%, 10%, 12%, 4-20%, 4-12%, 14%, 16%, 18%, 8-16%, 10-20% 4-12%, 8%, 10%, 12% (Bis-Tris)
3-8%, 7% (Tris-Acetate)
Price / Gel
(US List Price)		 NA $7.80 $13.17
Pack Sizes Available NA 5 & 10 packs 2 & 10 packs
View more
Order Now Order Now Order Now

Recommended Well Loading Volumes and Sample Loads

Specialized Protein Separation Gels

Life Technologies also offers a variety of gels for specialty applications including the following:

  • High throughput
  • Native protein analysis
  • Low MW protein separation
  • Protease separation
  • 2D Electrophoresis

View all specialized protein separation gels

  • Youtube
  • Twitter
  • Facebook