Protein Gels | Life Technologies

Protein Gels

The first step in protein analysis is sample separation. Protein gel electrophoresis is the most common technique used to separate proteins from cell and tissue extracts, blood or other biological sources. Traditionally, protein gels had to be poured just prior to the electrophoretic run and the steps involved in their creation could be time-consuming and could also introduce artifacts in the experiment because of large variations in the gel composition.

Using gels from another company? Use our product selection guide to find the Life equivalent!

Figure 1. Migration patterns of Mark12™ Unstained Standard on NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, and Bolt™ Gels. For optimal results, protein bands should migrate within the yellow shaded areas.

Migration patterns of protein standards on NuPAGE® Novex Gels

Which Protein Gel Is Right for You?

	Pour your own essentials Preassembled, leak-free empty cassettes	Pre-cast convenience with Laemmli chemistry	Highest resolution, longest shelf-life and western optimized gels	Highest resolution and long shelf-life
	Gel Casting Accessories	Novex® Tris-Glycine Express Kit	Bolt™ Gels	NuPAGE® Gels
Citations in the last 10 years	N/A	>5,000	N/A (new product)	>20,000
Protein resolution	Low	Medium	High	High
Lot-to-lot consistency	Low	Medium	High	High
Protein integrity	Low	Medium	High	High
Band quality	Low	Medium	High	High
Average run time	90 min	90 min	35 min	35 min
Well format	Standard	Standard	New high capacity wedge design	Standard
Gel dimensions	Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick	Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick	Mini: 8x8.3 cm, 1 mm thick	Mini: 8x8 cm, 1 or 1.5 mm thick Midi: 8 x 13 cm, 1 mm thick
Shelf life	N/A	1 - 2 months	>16 months	8 months (Tris-Acetate) 16 months (Bis-Tris)
Pack sizes	N/A	5 & 10 packs	10 packs	2 & 10 packs
Price / gel (US list price)	N/A	$7.80	$10.30*	$13.17
Gel % available	N/A	4%, 6%, 8%, 10%, 12%, 4-20%, 4-12%, 14%, 16%, 18%, 8-16%, 10-20%	8%, 10%, 12%, 4-12%	4-12%, 8%, 10%, 12% (Bis-Tris) 3-8%, 7% (Tris-Acetate)
Max protein load / band for optimal resolution	N/A	0.5 µg	0.5 µg	0.5 µg
Separation range	6 - 500 kDa	6 - 500 kDa	1.5 - 300 kDa	1.5 - 300 kDa (Bis-Tris) 30 - 400 kDa (Tris-Acetate)
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*Introductory pricing

Specialized Protein Separation Gels

Life Technologies also offers a variety of gels for specialty applications including the following:

High throughput
Native protein analysis
Low MW protein separation
Protease separation
2D Electrophoresis

View all specialized protein separation gels