Multiplexed Proteomics™ Technology

Protein Characterization

Multiplexed Proteomics™ Technology for Protein Profiling Analysis

The Molecular Probes™ Multiplexed Proteomics™ Technology is designed to directly visualize differential phosphorylation or glycosylation patterns, compared to total protein, in the same gel. The Multiplexed Proteomics™ Kits contain either Pro-Q® Diamond Phophoprotein Stain or Pro-Q® Emerald Glycoprotein Stain and SYPRO® Ruby Total Protein Stain. Phosphoproteins or glycoproteins are stained and imaged relative to total protein in the same gel. This allows unambiguous matching of proteins bands or spots and relative quantitation of differential phosphorylation or glycosylation patterns. Differences in posttranslational modifications relative to total protein expression can be distinguished in complex samples by two-dimensional gel electrophoresis, or multiple sample fractions can be screened in order to locate proteins of interest. Multiplexed Proteomics™ Kits include either phosphoprotein or glycoprotein standards which serve as molecular weight standards and positive or negative controls for specific post translational protein staining.


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