SDS-PAGE Buffers and Reagents

SDS-PAGE electrophoresis is one of the most common methods for protein separation. Invitrogen offers premixed, reliable Novex® SDS-PAGE buffers and reagents including sample buffers, running buffers, protein gel transfer buffers as well as reducing agents and antioxidants. Use the selection guide below to find the optimal buffer system for your experiments.

Precast SDS-PAGE Protein Gels

Recommended SDS-PAGE Buffers & Reagents

Gel TypeSample Buffer & ReagentsRunning BufferTransfer BufferBuffer Kit*
NuPAGE® Bis-Tris Gel• NuPAGE® Sample Reducing Agent (10X)
• NuPAGE® Antioxidant
• NuPAGE® LDS Sample Buffer (4X)		• NuPAGE® MES SDS Running Buffer (20X)
• NuPAGE® MOPS SDS Running Buffer (20X)		NuPAGE® Transfer Buffer 20X• NuPAGE® MES SDS Buffer Kit
• NuPAGE® MOPS SDS Buffer Kit
NuPAGE® Tris-Acetate Gel• NuPAGE® Sample Reducing Agent (10X)
• NuPAGE® Antioxidant
• NuPAGE® LDS Sample Buffer (4X)		NuPAGE® Tris-Acetate SDS Running Buffer (20X)NuPAGE® Transfer Buffer 20XNuPAGE® Tris-Acetate SDS Buffer Kit
Novex® Tris-Glycine Gel
• Novex® Tris-Glycine SDS Sample Buffer(2X)
• NuPAGE® Sample Reducing Agent		Novex® Tris-Glycine SDS Running Buffer (10X) Novex® Tris-Glycine Transfer Buffer (25X) 
Novex® Tricine GelNovex® Tricine SDS Sample Buffer (2X)Novex® Tricine SDS Running Buffer (10X)Novex® Tris-Glycine Transfer Buffer (25X)Novex® Tricine SDS Sample Kit
*NuPAGE Buffer Kits contain indicated MOPS or MES Running Buffer, NuPAGE® Sample Reducing Agent, NuPAGE® Antioxidant, and NuPAGE® LDS Sample Buffer; Novex® Tricine Sample Kit contains Novex® Tricine SDS Sample Buffer and Novex® Tricine SDS Running Buffer
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Additional Resources

For Research Use Only.  Not intended for any animal or human therapeutic or diagnostic use.
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