Electrophoresis Buffers and Reagents
 | Finding the right protein gel electrophoresis system is critical to getting the consistent and accurate results you need for your protein research. Reagents and buffers are important components of the electrophoresis system. Invitrogen offers a range of Novex® reagents and buffers for gel casting and sample preparation to running, staining and drying gels. |
Get Started Today With Buffers & Reagents For:
Gel Casting Buffers & Reagents
 | Life Technologies offers high quality and reliable reagents and buffers for pouring your own polyacrylamide gels, including urea, acrylamide, TEMED, and Rhinohide™ gel strengthner. We also offer Novex® gel cassettes and combs to make it even easier. Not sure you want to pour your own gels? No problem. Learn more about our extensive line of Precast Protein Gels that can help you get your experiments done faster and more efficiently. |
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Gel Electrophoresis Buffers and Reagents
Below you will find our recommendations for sample preparation, running buffers and other reagents for each type of protein gel electrophoresis systems that we offer including native gel electrophoresis, zymography, and isoelectric focusing.
- Find buffer and reagent recommendations for SDS-PAGE Gel Electrophoresis
- Find buffer and reagent recommendations for 2D- Gel Electrophoresis
Native Electrophoresis Recommended Buffers & Reagents
| Gel Type | Sample Preparation Reagents | Running Buffer | Transfer Buffer | Buffer Kit* |
|---|---|---|---|---|
| Novex® Tris-Glycine Gel | • Tris-Glycine Native Sample Buffer (2X) | • Novex® Tris-Glycine Native Running Buffer (10X) | • Novex® Tris-Glycine Transfer Buffer (25X) | |
| NativePAGE™ Bis-Tris Gel | • NativePAGE™ Sample Buffer (4X) • NativePAGE™ 5% G-250 Sample Additive | • NativePAGE™ Running Buffer (20X) • NativePAGE™ Cathode Buffer Additive (20X) | • Novex® Tris-Glycine Transfer Buffer (25X) | • NativePAGE™ Sample Prep Kit • NativePAGE™ Running Buffer Kit |
| *NativePAGE™ Sample Prep Kit—contains NativePAGE™ Sample Buffer (4X), 5% G-250 Sample Additive, 10% DDM, and 5% digitonin; NativePAGE™ Running Buffer Kit—contains NativePAGE™ Running Buffer (20X) and NativePAGE™ Cathode Buffer Additive (20X) | ||||
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Zymography Gel Recommended Buffers & Reagents
| Gel Type | Sample Buffer | Running Buffer | Gel Development Buffer | Gel Renaturing Buffer |
|---|---|---|---|---|
| Novex® Zymogram Gel | Novex® Tris-Glycine SDS Sample Buffer (2X) | Novex® Tris-Glycine SDS Running Buffer (10X) | Novex® Zymogram Development Buffer (10X) | Novex® Zymogram Renaturing Buffer (10X) |
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IEF Gel Recommended Buffers
| Gel Type | Sample Buffer | Running Buffer | Buffer Kit* |
|---|---|---|---|
| Novex® IEF Gel | • Novex® IEF Sample Buffer, pH 3–10 (2X) • Novex® IEF Sample Buffer, pH 3–7 (2X) | • Novex® IEF Anode Buffer (50X) • Novex® IEF Cathode Buffer, pH 3–10 (10X) • Novex® IEF Cathode Buffer, pH 3–7 (10X) | • Novex® pH 3-10 IEF Buffer Kit • Novex® pH 3-7 IEF Buffer Kit |
| *Novex® pH 3-10 IEF Buffer Kit--contains Novex® IEF Anode Buffer (50X), Novex® IEF Cathode Buffer pH 3-10 (10X), and Novex® IEF Sample Buffer pH 3-10 (2X); Novex® pH 3-7 IEF Buffer Kit--contains Novex® IEF Anode Buffer (50X), Novex® IEF Cathode Buffer pH 3-7 (10X), and Novex® IEF Sample Buffer pH 3-7 (2X) | |||
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Gel Staining and Drying Solutions and Reagents
Below are our gel drying solutions and a select number of our gel staining reagents. Learn more about our Protein Gel Staining and Imaging products.
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Novex® Gel Cassette Video
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
