Protein Expression & Production
 | Life Technologies offers a range of protein expression systems and tools that are easy to use,deliver high protein yields for every downstream application and allow you to get there quickly. Creating your gene, purifying your plasmid and delivery is simplified with the selection tools and educational videos. |
On This Page
Overview | Choosing Expression | Additional Information |
Selecting a Host System for Protein Expression & Production
When selecting a host system for expressing protein there are various considerations. From post translational modifications, equipment available to how quickly you need it, we have mapped out the hosts to help you make the choice. For each host system we have a information that provides you with all our applicable products along the we offer, allowing you to make the right choice based on your needs and budget.
| Do you need modifications? | How quickly do you require protein? | What capabilities do you have to support expression (lab equipment & resources)? | |||||||
|---|---|---|---|---|---|---|---|---|---|
| None | Some | Complete | Days | Weeks | Months | None | Some | Many | |
| Mammalian |  | | | ||||||
| Bacteria |  | | | ||||||
| Yeast |  | | | ||||||
| Insect |  | | | ||||||
Mammalian Expression
| Vector selection | Cloning | Transform in E.Coli | Plasmid Purification | Transfection | Expression | Purification | Analysis |
Determined by promoter and terminator | Determined by vector choice - or - | Recommend DH5alpha TOP10 strain | Could be maxi, mega or giga | Depends on speed | Media based on expression system choice: 293 or Freestyle | Affinity based with tag or native | Analysis, & Assay |
Bacterial Expression
| Vector selection | Cloning | Transform in E.Coli | Plasmid Purification | Transform | Optimization of Clone | Scale & Purify | Analysis |
Any vector with a T7 promoter | - or - | Recommend TOP10 strain but can choose from subcloning products | Mini prep kits | Transform into any expression strain | TB or LB | TB, LB or MagicMedia & resin purification | Analysis, & Assay |
Yeast Expression
| Vector selection | Cloning | Transform in E.Coli | Plasmid Purification | Transform Into Yeast | Clone Selection | Express | Purification | Analysis |
Does protein have a secretion signal Yes? use Pichia® | Clone with restriction enzymes | One Shot® TOP10 Electrocomp™ E. coli | Yeast strain | Zeocin | Media | With secretion sometimes not necessary & affinity for tag | Analysis & Assay |
Insect Expression
| Vector selection | Cloning | Transform in E.Coli | Plasmid Purification | Transform Into Bac | Purify Bacmid | Transfect | Amplify/ Express | Analysis |
Choose TOPO or Gateway vector | Select TOPO or Gateway for Cloning | Recommend DH5alpha TOP10 strain but can choose from subcloning CVC | Mini prep kits | Transform into DH10Bac | Midi or maxi prep- depending on yield requirement | Transfection with CellFectin II | SF9 & SF21 | Analysis, & Assay |
How-To Videos
Cloning | Transformation | |||
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Plasmid Purification | Expression | |||
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"Meet the Inventor" Series
Promotions
Improve protein expression up to 100-fold with GeneArt® expression-optimized genes.
Use promo code P909941 (US) or P909945 (Canada)
| Product | Size | Cat. No. | header |
|---|---|---|---|
| One Shot® TOP10 Chemically Competent E. coli | 40 reactions | C404006 | Buy now |
| One Shot® TOP10 Chemically Competent E. coli | 20 reactions | C404003 | Buy now |
| One Shot® Mach1™ T1 Phage-Resistant Chemically Competent E. coli | 20 x 50 µl | C862003 | Buy now |
Use promo code P909821 (US) or P909822 (Canada)
| Product | Size | Cat. No. | header |
|---|---|---|---|
| PureLink® HiPure Plasmid Filter Midiprep Kit | 25 preps | K210014 | Buy now |
| PureLink® HiPure Plasmid Filter Maxiprep Kit | 10 preps | K210016 | Buy now |
Use promo code P909894 (US) or P909900 (Canada)
| Product | Size | Cat. No. | header |
|---|---|---|---|
| BenchPro® 2100 Plasmid Processing Station | 1 each | MC1001 | Buy now |
| BenchPro® 2100 Air Compressor | 1 unit | MC5001 | Buy now |
| BenchPro® 2100 Air Compressor (Oil Filled) | 1 unit | MC6001 | Buy now |
Save big on protein expression media with these Gibco® Promotions
