pcDNA™3.3 TOPO® Mammalian Expression Vector
Powerful mammalian expression vector delivers high protein yields
The pcDNA™3.3-TOPO® TA Mammalian Expression Vector enables expression of exceptionally high levels of recombinant protein in adherent-or suspension-adapted mammalian cells.
Ideal for large-scale protein production
Figure 2. High Yields of Recombinant Protein. We used pcDNA™3.3-TOPO® vector to clone and express milligram levels of erythropoietin (EPO), Factor IX (FIX), and IgG in FreeStyle™ CHO-S cells. pcDNA™3.3-TOPO® constructs containing the relevant PCR-derived gene sequences were transfected into FreeStyle™ CHO-S cells in 30 ml volumes. Heavy and light chain genes were co-transfected for IgG expression. Four to six days posttransfection, EPO and FIX were quantified using immobilized goat anti–human IgG antibody. Each bar shows the average of two expression runs.
Figure 3. pcDNA™3.3 vector map. The latest version in the pcDNA vector family, pcDNA™3.3 offers you the flexibility to express a native protein or add your favorite purification tag to the gene of interest prior to TOPO® cloning.
Our pcDNA™3.3 vector incorporates TOPO® cloning technology, which removes time-intensive and unreliable steps from your cloning workflow, allowing you to perform bench-top cloning reactions in just 5 minutes. With up to 95% recovery of your desired clone, you always have the clone you need for downstream experiments.
Five-Fold Better Expression
We cloned and measured expression of luciferase (panel A) and ß-galactosidase (panel B) in pcDNA™3.3-TOPO® vector, pCI and pCMV-Script® Vectors. After transient transfection into adherent GripTite™ 293 MSR cells, ß-galactosidase activity and Luciferase activity is 2 and 5-fold higher, respectively, using pcDNA™3.3 Vector compared to competitor vectors. Error bars shown represent standard deviation (n = 6).