Scalable Transient Protein Expression Systems
 | Mammalian transient expression systems enable flexible and rapid production of proteins and are ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more native folding and post-translational modifications, such as glycosylation, than expression systems based on hosts such as E. coli, yeast, or insect cells. |
Choose which 293-Based System is Right for You
| FreeStyle™ 293 System | FreeStyle™ MAX 293 System | Expi293™ System | |||
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The most widely used transient expression system
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Novel FreeStyle™ MAX reagent used for both 293 and CHO transient transfection
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Highest protein yields, most cost effective system
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| Cells | FreeStyle 293-F | FreeStyle 293-F | Expi293F | ||
| Media | FreeStyle 293 Medium | FreeStyle 293 Medium | Expi293 Medium | ||
| Transfection reagent | 293fectin | FreeStyle MAX | ExpiFectamine 293 Transfection Kit | ||
| Positive control vector | pCMV Sport β-gal | pCMV Sport β-gal | Antibody-Expressing Vector | ||
| Maximum Working Culture Density | 2x106/mL | 2x106/mL | 6x106/mL | ||
| Cell Density for Transfection | 1 x 106/mL | 1 x 106/mL | 2.5 x 106/mL | ||
| Protein Yield* | 20 – 200 mg/L | 20 – 200 mg/L | 200 – 1000 mg/L | ||
| Total cost to grow and transfect 1L of culture** | $1310 | $1122 | $1531 | ||
| Order Now |
** Total cost of media, transfection reagent and plasmid DNA to grow cells and transfect 1 L of culture
FreeStyle™ MAX CHO Expression System
- Large-scale protein expression in CHO cells
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