Jump-In™ Cell Engineering Platform
 | Whether you are an experienced cell engineer, or need engineered cells for your projects, the Jump-In™ cell engineering platform can accelerate your projects by enabling you to generate engineered cell lines in much less time than traditional methods. The Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase. Unlike the better-known recombinases such as Cre and Flp, PhiC31 integrase catalyzes recombination between two nonidentical sites and lacking a corresponding excision enzyme makes the integration unidirectional and virtually irreversible. In addition, combining PhiC31 integrase with R4 integrase allows for isogenic expression from a defined genomic locus, providing the ideal solution for comparative analysis of gene families, isoforms or orthologs. Depending on your needs and experience, we offer multiple options for accessing Jump-In™ technology, allowing you to choose the method that best utilizes your available resources and fits your desired timelines. |
Quote from a US Pharmaceutical Company Researcher
 | [Jump-In] technology has been deployed quite broadly and successfully here. It's fast and efficient. One scientist can make several stable cell lines simultaneously; and [we see] even receptor expression across targets and across species orthologues. |  |
| Jump-In™ Fast Gateway® System | Jump-In™ TI™ Gateway® System | Jump-In™ Parental Cell Line Kits | Jump-In™ Custom Services | |
| Saves time and effort compared to traditional cell engineering methods | Yes | Yes | Yes | Yes |
| Engineered cell line is isogenic | No | Yes | Yes | Choice |
| Can use your preferred cell background | Yes | Yes | Limited to parental cell lines in catalog | Choice |
| Requires generation of platform/parental cell line (with R4 sites) | No | Yes | No | Choice |
| Requires clonal selection | Optional | Yes | Optional | Choice |
| Ideal when you need… | Faster stable cell lines in your preferred cell background | Isogenic cell lines in your preferred cell background | Isogenic cell lines created with less hands-on time | Experts who can meet your project specifications and timelines |
Jump-In™ Fast Gateway® System
Jump-In™ Fast Gateway® System allows you to generate stable clones in just two weeks by introducing your genetic material into your cell background using PhiC31 integrase to produce a highly positive pool of retargeted cells. Screen only 10 clones to find one with your ideal expression level.
Jump-In™ TI™ Gateway® System
Jump-In™ TI™ Gateway® System enables the creation of isogenic cell lines that express your gene of interest by having you initially generate a retargetable platform cell line containing the R4 attP sites in your preferred cell background.
Jump-In™ Parental Cell Line Kits
Jump-InTM parental cell line kits allow you to generate isogenic cell lines by providing a parental (platform) cell line with a single R4 attP site ready to be retargeted with your genetic material.
Jump-In™ Custom Services
Reduce assay development and cell engineering project timelines by outsourcing your cell line development to our Custom Services team.
Let us craft a Jump-InTM parental cell line or the final engineered cell line meeting your defined specifications; while a dedicated project manager provides frequent updates on project milestones.
For more information on our stable cell line generation custom services, email us at: discoveryservices@lifetech.com.
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