Fluorescent Protein Vectors

These Vivid Colors™ vectors are for generation of N-terminal or C-terminal Fluorescent Protein fusions to your Gene-Of-Interest (GOI). Emerald Green (EmGFP) and Yellow (YFP) Fluorescent Protein versions are available. To create a Vivid Colors™ fusion with protein, you can employ either Gateway® or TOPO®-cloning technology. Gateway® Destination Vectors require the use of an entry clone, while the TOPO® vectors require only a PCR product of your GOI (T Cloning®). Regardless of the cloning techniques you select, the Ultimate ORF™ collection is a comprehensive and convenient source of full-length cDNA clones for creating fusion proteins.

Co-expression of Your Protein with a Vivid Colors™ Fluorescent Protein

Express your protein in it’s native-protein configuration AND EmGFP from a single vector. The Vivid Colors™ pcDNA™ 6.2/ EmGFP-Bsd / V5-DEST vector allows you to rapidly identify transfected cells to look for biological activity conferred by your gene. You’ll need to use a Gateway® entry-clone version of your gene to clone into this destination (DEST) vector. The Ultimate ORF™ collection is a comprehensive and convenient source of full-length entry clones for this purpose. If you already have your a clone of your protein, you can amplify it by PCR and clone it with TOPO®-technology to prepare the entry-clone.

Create Your Own Vectors with Other Vivid Colors™ Fluoresecent Proteins

The pRSET/CFP (cyan) and pRSET/BFP (blue) vectors contain alternative color versions of fluorescent proteins derived from Aequorea victoria. These proteins are commonly used with the YFP and EmGFP for multi-color applications. Configured for bacterial expression and fluorescent protein purification, these vectors can also be used for subcloning to construct customized mammalian expression vectors.

Lentiviral (Vivid Color™) Emerald GFP Control Expression Vector

To optimize lentivial packaging and transduction efficiencies and monitor infected cells, you need the pLenti 6.2/EmGFP-GW Expression Control Vector. This vector allows you to generate high-titer Lentiviral preps that express EmGFP and provides for blasticidin selection. To learn more about the Lentiviral Expression product line visit our ViraPower™ catalog pages.

Other Fluorescent Protein Vectors (Cycle 3 Green Fluorescent Protein)

To express your gene with alternative promoters, or with other selectable markers for stable-cell generation, consider the pTracer vectors series. These vectors express the Cycle 3 Green Fluorescent Protein (Cycle 3 GFP) which has spectral excitation and emission wavelengthsidentical to wild-type GFP. Although nearly as bright as the Vivid Colors™ FPs, you’ll need to use the proper filter set to observe the fully-enhanced brightness.

• pTracer™/Bsd Expression Vectors
  Choose from CMV or EF-1 a promoters for expressing your gene of interest. The pTracer™ EF vector will allow expression of your protein with carboxy-terminal V-5 and 6xHis epitope tags. The pTracer™ CMV vector is for expression of your protein without any epitope tags. These pTracer™ /Bsd Vectors express the blasticidin resistance gene as a fusion product with the Cycle 3 GFP.
• pTracer™/Zeo Expression Vectors
  Choose from SV40, CMV or EF-1 a promoters for expressing your gene of interest. The pTracer™ EF vector will allow expression of your protein with carboxy-terminal V-5 and 6xHis epitope tags. The pTracer™ SV40 and CMV vectors are for expression of your protein without any epitope tags. These pTracer™ /Bsd Vectors express the zeocin resistance gene as a fusion product with the Cycle 3 GFP.
• Promoter Characterization
  If you’re trying to characterize an unknown promoter, you’ll need a cloning and reporter system to monitor expression efficiencies from your PCR-generated mutants. The pGlow and pBlue TOPO® TA Expression Kits provide the convenience of TOPO®-cloning of your PCR products, and the option to quantify promoter activity with either Cycle 3 GFP (pGlow) or b - galactosidase (pBlue) expression .
• GFP (cycle 3) TOPO® TA Expression Kits
  These vectors are for cloning your PCR-amplified gene of interest to generate a fusion protein with Cycle 3 GFP fused on either the N- or C-termini. With TOPO®-cloning, only a 5-minute reaction is required to generate a clone. Following verification of orientation, the fusion protein is ready to express. The vector elements for these TOPO®-cloning vectors include a CMV promoter for high-level expression of your gene of interest and a neomycin resistance marker.

Highly Specific Anti-GFP Antibodies for Vivid Colors™ Fluorescent Proteins
Highly specific antibodies to Aequorea victoria fluorescent proteins are available, including polyclonal Anti-GFP (rabbit serum) and Anti-GFP (rabbit IgG fraction), for Western Blot detection. These antibodies have been tested and are shown to react specifically and strongly to both Vivid Colors™ EmGFP and YFP. A number of different conjugated forms of these antibodies are also available from Molecular Probes, offering you a choice of multiple detection technologies you can employ.

Anti-GFP; Rabbit polyclonal antiserum (A6455)
Anti-GFP; Rabbit polyclonal IgG fraction (A11122)
Anti-GFP; ABfinity™ recombinant rabbit monoclonal antibody (G10362)