BaculoDirect™ Baculovirus Expression System
Traditional baculovirus expression systems utilize tedious and time consuming site-specific transposition in E. coli or lengthy homologous recombination in insect cells to generate the recombinant baculovirus. In contrast, BaculoDirect™ Baculovirus Expression System uses a quick 1 hour Gateway® recombination reaction to produce the necessary bacmid for transfection, saving days to produce recombinant baculovirus.
Fastest method to generate recombinant baculovirus
BaculoDirect™ Method Overview
Engineered for expression
The BaculoDirect™ linear DNA is designed for simple generation of recombinant baculovirus and expression insect cells. In addition to attR site for quick Gateway® recombination cloning, the backbone contains strong polyhedrin promoter for high protein expression and a C-terminal or N-terminal 6xHis and V5 tag for detection and purification. Show here at right is Western blot analysis of 5 proteins cloned into and expressed using the BaculoDirect™ system.