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Protein Expression & Analysis

Cell-Free Expression

Overview of the Expressway™ Cell-Free Expression System

The Expressway™ Cell-Free Expression Systems utilize an efficient, coupled transcription and translation reaction to produce up to milligram quantities of active recombinant protein in a tube reaction format. The Expressway™ systems eliminate the time-consuming steps of traditional cell-based protein production such as transformation, cell culture maintenance, and expression optimization. In just a few hours, high-yield and high-quality protein suitable for an array of downstream functional and structural studies can be produced with this E. coli-based in-vitro system. The recent improvements to the Expressway™ Cell-Free Expression System with the slyD mutant E.coli. extract and patented feed buffer produces the highest level of active proteins.
The improved Expressway™ Cell-Free Expression Systems are available in four formats for different applications (Table 1). Whether you’re making protein from plasmid or linear DNA, for rapid expression screening or large amount protein production, or for downstream structural or functional analyses (see references below), Expressway™ Cell-Free Systems can greatly simplify your protein expression.

Table 1. Expressway™ Cell-Free Expression Systems and Applications

Application Product*
Rapid, small-scale protein expression starting from plasmid or linear DNA template Expressway™ Mini Cell-Free Expression System
Rapid, small-scale protein expression with direct N-terminal or C-terminal Lumio™ tag detection, starting from plasmid or linear DNA template Expressway™ Lumio™ Expression System
Rapid, milligram-scale protein expression, or high- throughput protein expression screening, starting from plasmid or linear DNA template Expressway™ Maxi Cell-Free E. coli Expression System
Rapid, milligram-scale protein expression for highly efficient labeling for protein NMR analysis, starting from plasmid or linear DNA template Expressway™ NMR Cell-Free E. coli Expression System
*For customers who used Expressway™ Systems prior to March 1, 2006, please click here or contact Technical Services at 800 955 6288 Ext.2 to find a comparable product that provides optimal protein synthesis for your application.