Cell-Free Expression

Fast and Easy Cell-Free Expression of Functional Proteins

In vitro expression systems use an efficient coupled transcription and translation reaction to produce high yields of full-length, functional protein from E. coli cell lysates. The time-consuming steps of cell-based protein production  have been eliminated; now, in only two hours, you can produce protein starting from plasmid or linear DNA.

Methods

Simple, Rapid Cell-Free Expression

Go from plasmid to protein in two hours with Expressway™ Mini kits

Milligram Scale Cell-Free Expression

Produce milligram quantities of protein starting from plasmid or linear DNA with Expressway™ Maxi kits

Applications

Fluorescent Labeling

Quickly and efficiently produce N-terminal or C-terminal–tagged fluorescent proteins with Expressway™ Lumio™ Kits

Cell-Free Membrane Protein Expression

Cell-free expression for maximum yield and convenience—MembraneMax™ kits

Resources

References

           		Brochures and Posters