Learn About Scalable Transient Protein Production in Mammalian Cells
Who Should Attend
- Scientists needing to quickly produce milligram to gram quantities of recombinant protein in mammalian cells
- Scientists, Managers and Lab Directors establishing core protein production facilities
- Bioproduction and Process Development scientists wanting to learn about transient protein production methods
- Scientists generating recombinant proteins by stable cell expression desiring a much faster method to obtain recombinant proteins
- Protein expression scientists looking to move into mammalian systems
The class reviews the four essential elements of any mammalian transient production system: cell lines, expression vectors, transient transfection and cell culture.
The course helps participants understand differences and tradeoffs in producing recombinant proteins in HEK293 or CHO cells. Attendees will receive both classroom and hands-on instruction in protocols for small to large-scale transient transfection of suspension cells. In addition to areas such as; optimization and monitoring of culture conditions; optimizing and measuring transfection performance.
The workshop will review the equipment needed to establish a transient production facility and help provide an understanding of what parameters play the most critical roles in obtaining the highest level of protein expression.
We will also feature the BenchPro® 2100 Plasmid Purification System, delivering transfection-grade plasmid DNA for all of your downstream application needs with less than 5-minute setup time.