Organelle Isolation
 | Dynabeads® can been used to isolate a number of different organelles and organelle-specific proteins. The magnetic separation technology enables specific isolations of true fractions in small volumes, and can replace time-consuming and labor intensive density gradient centrifugations. |
Advantages incude:
- Rapid, high-purity isolations from crude fractions or fractions of common densities
- Morphologically intact target organelles - can be fixed for EM studies or solubilized directly on the bead surface
- Immunomagnetically isolated organelles can be assayed by all conventional techniques
- Easy protocol yields high-purity functional organelles of interest
As more and more organelle specific markers are identified, corresponding immunomagnetic isolation of subgroups of organelles can be performed. Examples of organelles or organelle fractions that have been isolated with Dynabeads® include: exosomes, mitochondria, peroxisomes, kidney glomeruli and endondocytic and secretory compartments. Click to see a list of selected references citing the use of Dynabeads®
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| 1. Select your application | Application specification | 2. Click on the Solution | Advantage |
| Isolation of fragile organelles or organelle fractions in general | Using a mouse primary antibody | Dynabeads® M-280 Sheep anti-Mouse IgG | Direct or indirect isolation of organelles made more efficient with two layers of Abs. |
| Using a rabbit primary antibody | Dynabeads® M-280 Sheep anti-Rabbit IgG | Direct or indirect isolation of organelles made more efficient with two layers of Abs. | |
| Antibodies made in any other species | Dynabeads® Protein G Dynabeads® Protein A | Efficient direct or indirect isolation of organelles. | |
| Isolation of large organelle fractions or isolation from a viscous sample | Using antibodies made in a specific species | Dynabeads® M-450 with a secondary antibody against the corresponding primary Ab species | More efficient pull to the magnet in viscous samples |
| Isolation of organelles using a ligand that is not an antibody | When high yield is important | Dynabeads® M-280 Tosylactivated | Easy coupling of a molecule onto these hydrophobic beads for efficient organelle isolation |
| Isolation of organelles using a ligand that is not an antibody | When high purity is important | Dynabeads® M-270 Epoxy | Coupling of a molecule onto these hydrophillic beads for very high purity organelle isolation |
In addition to the products listed above, the larger 4.5 µm Dynabeads® can also be used: Dynabeads® Sheep anti-Mouse IgG, Dynabeads® Goat anti-Mouse IgG, Dynabeads® Sheep anti-Rat IgG, Dynabeads® Rat anti-Mouse IgG1, Dynabeads® Rat anti-Mouse IgM and Dynabeads® Pan Mouse IgG
