NovaBright™ Reporter Gene Assay Selection Guide
| NovaBright™ Dual Beta-gal and Firefly | Luciferase System | NovaBright™ SEAP System | |
| Reporter Enzyme(s) | Beta-galactosidase | Firefly luciferase, beta-galactosidase | Secreted placental alkaline phosphatase |
| Key Attributes | Homogeneous assay does not require removal of culture medium | Detection of luciferase and beta-galactosidase from the same cell extract | No cell lysis required |
| Substrate | Gallacton-Star® | Luciferin Galacton-Plus® | CSPD® |
| Duration of Light Emission | 60–90 min | 60–90 min | 1–2 hr |
| Reagent Injection | Not required | Required | Not required |
| Special Features | Designed for assays on cells cultured in luminometer plates for high-throughput screening. Choice of reaction buffers for lysis of mammalian or yeast and mammalian cells. | Two assays from one cell extract enables better precision for normalizing transfection efficiency. | Cells remain viable; useful for stable transfectants, time course studies, etc. |
| See all Beta-gal Kits | See all Luciferase Kits | See all SEAP Kits |