NovaBright™ Reporter Gene Assay Selection Guide

 NovaBright™ Dual Beta-gal and Firefly Luciferase SystemNovaBright™ SEAP System
Reporter Enzyme(s)Beta-galactosidase Firefly luciferase, beta-galactosidaseSecreted placental alkaline phosphatase
Key AttributesHomogeneous assay does not require removal of culture mediumDetection of luciferase and beta-galactosidase from the same cell extractNo cell lysis required
SubstrateGallacton-Star®Luciferin Galacton-Plus®CSPD®
Duration of Light Emission60–90 min60–90 min1–2 hr
Reagent InjectionNot requiredRequiredNot required
Special FeaturesDesigned for assays on cells cultured in luminometer plates for high-throughput screening. Choice of reaction buffers for lysis of mammalian or yeast and mammalian cells.Two assays from one cell extract enables better precision for normalizing transfection efficiency.Cells remain viable; useful for stable transfectants, time course studies, etc.
 See all Beta-gal KitsSee all Luciferase KitsSee all SEAP Kits