ProtoArray® Kinase Substrate Profiling Service

Please fill out the information below to obtain more information and/or a quote for a Kinase Substrate Profiling Service project.
Fields marked with an * are required.

Contact Information

Courtesy Title

Mailing Address

City *

Job Role

  • CEO / COO / President
  • Vice President
  • Department Head
  • Principal Investigator
  • Medical Doctor
  • Director
  • Post-doctoral fellow
  • Scientist / Associate Scientist
  • Student / Graduate Student
  • Research Assistant / Lab Technician
  • Lab Manager
  • Purchaser / Procurer
  • Educator

Techniques

  • Mass Spectrometry
  • Cell Imaging
  • qPCR / Real-time PCR / qRT-PCR
  • DNA Sequencing
  • Next Generation DNA Sequencing
  • Nucleic Acid Purification and/or Separation
  • Transfection
  • Virus Isolation

Applications

  • BioMarker Discovery
  • Cell / Tissue Culture
  • Cloning
  • Environmental Testing
  • Epigenetics / Epigenomics Analysis
  • Food and Beverage Testing
  • Forensics / Human Identity
  • Gene Expression / RNA Analysis
  • Genotyping and Genetic Variation
  • Imaging & Microscopy
  • In Vivo Research
  • Plant Research
  • Protein Analysis / Proteomics
  • Protein Expression / Production
  • RNAi
  • Stem Cells

1) How many kinases will be tested?

2) Will you be using one of Invitrogen's kinases?

3) If provided by the customer, please list the kinase(s) that will be sent to Invitrogen for testing (optional).

4) If the kinase(s) will be provided by the customer, please list buffer components. (Note, ATP concentrations greater than 0.1nM may interfere with this assay)

5) What is/are the concentration(s) of your kinase(s)? (Invitrogen recommends >100nM.)

6) If known, what is the optimal working concentration of your kinase in your experiments?

7) What is the recommended storage condition of your kinase(s) (4°C, -20°C or -80°C)?

8) Have you tested your kinase(s) for activity?

  • Yes
  • No

9) The following buffer will be used for the ProtoArray™ Profiling Assays:

Please comment if there is reason to believe that your kinase(s) will not be active in the presence of these reagents

10) Does/Do your kinase(s) remain soluble in the presence of 150mM salt buffer?

Yes
No
Unknown

11) Does/Do your kinase(s) require the presence of a co-factor for activity? (must be provided by customer)

Yes
No
Unknown

10) Please specify the name(s) and concentration of co-factor(s)

11) Would you like to test the inhibition properties of compounds against your kinase(s)?

  • Yes
  • No

12) How many compounds will be submitted?

13) What is the optimal working concentration for your compound(s)? If unknown please send at least 50ml of 1 mM compound in solvent

14) Please list the solvents/buffers in which the compounds are dissolved (e.g. 100% DMSO).

15) Please list any special handling requirements for the inhibitor(s) prior to performing the assay, e.g. does your compound come out of solution?

Would like a quote or additional information?

  • Yes
  • No
For the ProtoArray® Kinase Inhibition Profiling Service, please send at least 150µl (at 1 µM) of your kinase on dry ice, at least 50µl of 100 µM compound and at least 50µl of the solvent in which your compound is dissolved.

Ship to:
ProtoArray® Kinase Substrate Profiling Service
688 East Main Street
Branford, CT 06405

Please note: in order to ensure a quality service we request that you do not freeze-thaw your kinase. 
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