Overview of ProtoArray® Human Protein Microarrays
 | The ProtoArray® Human Protein Microarray is an advanced, high-content, functional protein microarray that enables rapid profiling of thousands of biochemical interactions in as little as one day. Researchers can use the ProtoArray® microarray to perform a variety of assays including biomarker identification, drug target discovery, enzyme substrate identification, antibody specificity profiling, and protein-protein interaction studies. Results can be readily measured with most commercial scanners, and the data can be analyzed using our free ProtoArray® Prospector software (Figure 1). |
) | Figure 1. (Left) The high-content human ProtoArray® Human Protein Microarray contains thousands of full-length human proteins for rapid profiling and easy biomarker discovery. (Right) The ProtoArray® Prospector software is a user-friendly tool that enables rapid data analysis and cross-group comparisons. |
Absolute Identification of Your Protein Targets with ProtoArray® Human Protein Microarray
Each ProtoArray® protein array has over 9,000 human proteins printed in duplicate on a one inch by three inch slide. The proteins are produced from clones selected from the Invitrogen Ultimate™ ORF Collection, the first and only clone resource to offer full-insert sequenced clones in a Gateway® vector. Proteins are expressed as N-terminal GST fusion proteins using a baculovirus expression system. After protein expression, proteins are purified under nondenaturing conditions. The proteins are printed at +4°C on an ultrathin layer of nitrocellulose to preserve native protein structure without the need for chemical fixation. Each slide is labeled with a unique bar code for easy tracking.
Individual microarrays on nitrocellulose-coated glass slides allow for the greatest flexibility in experimental design, with protocols for each supported application available. Some of these profiling applications (Figure 2) include:- Immune Response Biomarker Profiling — Biomarker Discovery for rapid discovery of disease markers specific to a variety of infectious diseases, cancers, and autoimmune diseases
- Enzyme Substrate Profiling — Profiling of kinases, ubiquitin ligases, and methyltransferases for target discovery and validation
- Small Molecule Profiling — Profiling of radiolabeled, biotinylated, or fluorescently labeled small molecules for target identification or selectivity
- Protein-Protein Interaction Profiling — Protein-Protein Interactions for mapping biochemical pathways
- Antibody Specificity Profiling — Antibody specificity profiling for research and therapeutic antibody development
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Figure 2. Range of ProtoArray® applications. A. Biomarker discovery, B. Profiling enzymatic activity — Kinase substrate identification,methyltransferase substrate identification, and ubiquitin ligase profiling, C. Protein-protein interactions and antibody specificity profiling, and D. Small molecule protein profiling.
Profiling With ProtoArray® Protein Microarrays
Using the ProtoArray® Human Protein Microarray is simple — and you get high-quality results in a fraction of the time. Regardless of your study, fluorescent applications can be completed in as little as one day. ProtoArray® microarray applications follow the same simple workflow of incubating a sample on the array, probing with a detection reagent, and then visualizing with a fluorescent scanner or phosphorimager (Figure 3).
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Figure 3. Simple, robust assay for discovering novel protein-protein interactions in as little as one day.
