Antibody Specificity Profiling with ProtoArray®

Why profile your antibody cross reactivity

• Antibody specificity is crucial for the development of therapeutics.Cross reactivity can lead to serious unintended consequences.
  
  FDA Guidance
  
  "The Testing of Monoclonal Antibody Products for Human Use highlights the risk potential when the same or related antigenic determinant as targeted by the therapeutic antibody is expressed on human cells or tissues other than the intended target tissue."
  
  U. S. Department of Health and Human Services
  Food and Drug Administration
  Center for Biologics Evaluation and Research
  February 28,1997
  
  FDA suggests that ‘appropriate newer technologies should be employed as they become available and validated’.
  

Why use ProtoArray® protein arrays?

• ProtoArray® arrays are an excellent method to use for testing antibody specificity. Using this method you can profile your antibody against 9,000 functional proteins and identify possible cross reactivity. Since the identity of every protein on the array is known, you can immediately identify any proteins that your antibody binds to. The protocol is very straightforward and requires very little sample.
  

Protocol