Genotyping by Real-time PCR in Plants
 | Genotyping by real-time PCR, whether for the confirmation of single nucleotide polymorphisms (SNPs) or as a screen for panels of markers in hundreds or even millions of samples, is a well-known and demonstrated approach used widely in both research and industrial settings. Life Technologies is the leader in providing real-time PCR instruments, TaqMan® Assays, and reagents that generate reliable results for validation and screening. |
Flexible, high-throughput genotyping by real-time PCR with the QuantStudio™ 12K Flex Real-Time PCR System and Custom TaqMan® SNP Genotyping Assays
QuantStudio™ 12K Flex Real-Time PCR System
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Custom TaqMan® SNP Genotyping Assays Custom TaqMan® SNP Genotyping Assays provide a highly flexible technology for detection of polymorphisms within any genome. Create your own assays by submitting confidential target sequences to our secure assay design pipeline using the Custom TaqMan® Assay design tool. This pipeline has successfully generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data. Custom TaqMan® Assay Manufacturing & Plating Service Whether it’s zygosity analysis, or special manufacturing modifications such as a specific dye, assay volume/concentration, or plating format, Life Technologies can address your special needs. Contact your sales representative to discuss your needs. |
Together, the Custom TaqMan® SNP Genotyping Assays and QuantStudio™ 12K Flex system provide the flexibility and throughput you need for your project.
Have a sales rep contact meOverview of workflow for genotyping by real-time PCR
Publications using Custom TaqMan® SNP Genotyping Assays
| Title | Authors | Address | Link |
| Mapping of the oat crown rust resistance gene Pc91. | McCartney CA, Stonehouse RG, Rossnagel BG, Eckstein PE, Scoles GJ, Zatorski T, Beattie AD, Chong J | Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada. | View |
| A fluorogenic 5’ nuclease (TaqMan) assay to assess dosage of a marker tightly linked to red skin color in autotetraploid potato. | De Jong WS, De Jong DM, Bodis M | Department of Plant Breeding, Cornell University, Ithaca, NY 14853-1901, USA. | View |
| Discovery and characterization of single nucleotide polymorphisms in Chinook salmon, Oncorhynchus tshawytscha. | Clemento AJ, Abadía-Cardoso A, Starks HA, Garza JC. | Southwest Fisheries Science Center, National Marine Fisheries Service, Santa Cruz, CA 95060, USA. | View |
| SNP genotyping by the 5’-nuclease reaction: advances in high-throughput genotyping with nonmodel organisms. | Seeb JE, Pascal CE, Ramakrishnan R, Seeb LW. | School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, USA. | View |
| A nanoliter fluidic platform for large-scale single nucleotide polymorphism genotyping. | Roberts DG, Morrison TB, Liu-Cordero SN, Cho J, Garcia J, Kanigan TS, Munnelly K, Brenan CJ. | BioTrove Inc., Woburn, MA 01810, USA. | View |
| Simplex and duplex event-specific analytical methods for functional biotech maize. | Lee SH, Kim SJ, Yi BY. | GMO Testing Laboratory, Experiment Research Institute of National Agricultural Products Quality Management Service, Seoul, South Korea. | View |
| Quantitative real-time PCR assay for determining transgene copy number in transformed plants. | Biotechniques. 2001 Jul;31(1):132-4, 136-40. Ingham DJ, Beer S, Money S, Hansen G. | Syngenta, Research Triangle Park, NC, USA | View |
Ordering information
TaqMan ® GTXpress™ Master Mix and TaqMan® Sample-to-SNP™ Kit
| Product | Cat. No. |
Size |
TaqMan® GTXpress™ Master Mix |
400 reactions |
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4,000 reactions |
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2,000 reactions |
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40,000 reactions |
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TaqMan® Sample-to-SNP™ Kit |
200 mL sample prep, 10 mL PCR |
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20 mL sample prep, 10 mL PCR |
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20 mL sample prep, 50 mL PCR |
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5 mL sample prep, 1 mL PCR |
Custom TaqMan® SNP Genotyping Assays
| Product | Cat. No. |
Size |
Custom TaqMan® SNP Genotyping Assays |
Small: 1,500 reactions, 40x |
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Medium: 5,000 reactions, 40x |
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Large: 12,000 reactions, 80x |
Order Custom TaqMan® SNP Genotyping Assays now
Need help? Watch the custom TaqMan® Assay design tool tutorial
TaqMan® OpenArray® Genotyping Plates
| Plate Format | # of Samples/Plate | Min. Order | Total # of Samples/Min. Order |
| 16 assays | 144 | 10 | 1,440 |
| 32 assays | 96 | 10 | 960 |
| 64 assays | 48 | 20 | 960 |
| 128 assays | 24 | 40 | 960 |
| 192 assays | 16 | 60 | 960 |
| 256 assays | 12 | 80 | 960 |
Configure a TaqMan® OpenArray® Genotyping Plate
TaqMan® Custom Plating Service for 96-/384-well plates
Learn about our TaqMan® Custom Plating Service
