Plant DNA & RNA Isolation | Life Technologies

Plant DNA & RNA Isolation

Plant DNA Isolation has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA and RNA quality and inhibit downstream reactions. Life Technologies offers an expansive portfolio of kits, reagents, and devices for the isolation, purification, analysis, and quantitation not only for plant genomic DNA, but also for total RNA, mRNA, and microRNA or transcriptome RNA.

Jump to plant RNA Isolation

Which Plant DNA Isolation Kit is Right for You?

	Bulk buffers for crude extraction of DNA and RNA from plant cells (*)	DNA extraction and TaqMan® chemistry-based genotyping combined, <1h to results	The most efficient lysis for the largest amount of tissue	Most suitable for screening and GMO testing due to automation capabilities	Slica-based method for low abundance plant DNA samples	Magnetic beads designed to handle low-abundance plant DNA samples
	Total RNA Lysis Solution	TaqMan® Sample-to-SNP™ Kit	Plant DNAzol®	ChargeSwitch® gDNA Plant	PureLink™ Genomic Plant	MagMAX™ DNA Multi-Sample Kit
Top Seller
Starting Material	≥ 100 mg	2-3mm punch	≥ 100 mg	50-100 mg	100 mg	5-10 mg
Yield	varies based on starting material	varies based on starting material	varies based on starting material	up to 7 µg	up to 14.6 µg	up to 14.6 µg
Protocol time	<15 mins	5 mins	<60 mins	<15 mins	<40 mins	<40 mins
Plant sample types	Customer-validated for DNA on a broad range of plant tissues for SNP genotyping as well as corn flour for GMO testing	Works with broad rangeof plant tissues and starting material (leaf punch, needle, seed chips)	Broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass and Arabidopsis thaliana leaves)	Some of the many species and tissues are canola, corn, cotton, sunflower, barley, rice (seeds); tomato, cucumber, lettuce, banana (leaves); rose, magnolia (flower); Acer, sycamore, grass, etc.	Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass and Arabidopsis thaliana leaves)	Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass & Arabidopsis thaliana leaves)
Isolation technology	Bulk lysis buffer	Lysis solution	Organic extraction	ChargeSwitch® Chemistry, magnetic beads	Silica spin column	Magnetic beads
Downstream applications	PCR, real-time PCR	Real-time PCR	All	All	All	All
Automatable	no	yes	no	yes	no	yes
High throughput compatibile	yes	yes	yes	yes	no	yes
High throughput/ automation options	yes	yes	yes	ChargeSwitch® gDNA Plant 960 Kit	96-well plate	50 preps 96 preps
Cost/prep	$0.41	$1.29	$1.81	$2.56	$2.46	$2.82
Kit sizes available	250 mL	100 preps 400 preps 4000 preps	100 mL	96 preps 960 preps	50 preps 4 X 96 preps	50 preps 96 preps
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(*)The Total RNA Lysis solution was part of the discontinued ABI PRISM® 6100 Nucleic Acid PrepStation and is our cheapest and quickest methodology for plant DNA extraction without the use of organic solvents.

Which Plant RNA Isolation Kit is Right for You?

	Great for difficult samples (conifer tissue & seeds)	Rapid & fully automated	Quick & easy to use	Efficient recovery of microRNA & small RNA
	Plant RNA Reagent	MagMAX™-96 Total RNA Isolation Kit	PureLink® RNA Mini Kit	mirVana™ miRNA Isolation Kit
Top Seller
RNA types isolated	large RNA molecules only	large RNA molecules only	large RNA molecules only	Small & large RNA molecules
Prep time	60 minutes	<45 minutes	<20 minutes	30 minutes
Amount of starting material	Up to 1 g	Up to 10 mg	<250 mg	0.5 to 250 mg
Isolation method	High purity organic extraction (requires alcohol precipitation)	Scalable, flexible format with magnetic beads	Fast, convenient silica column	Highest purity & convenience; includes organic extraction & silica column (no precipitation required)
High throughput compatible
Compatible products		Ambion® Plant RNA Isolation Aid Removes common plant contaminants	Ambion® Plant RNA Isolation Aid Removes common plant contaminants	Ambion® Plant RNA Isolation Aid Removes common plant contaminants
Price per prep	$1.36	$3.00	$4.50	$6.65
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Other Products of Interest

In addition to DNA and RNA isolation products, Life Technologies offers an expansive portfolio of solutions for analysis and quantitation of plant genomic DNA.

Technical Resources

DNA

Extraction of Plant DNA Using Plant DNAzol® Reagent

RNA