Two-Step RT-PCR

The SuperScript® III First-Strand Synthesis System for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving you time and ensuring your success with every experiment.

  • Contains SuperScript® III RT for higher cDNA yields
  • Detect template molecules from as little as 1.0 pg of total RNA
  • Available in convenient SuperMix format for time-saving reaction setup 
  • View all Two-Step RT-PCR Kits

Better at 55°C

SuperScript III® RT is included to generate high yields of quality cDNA. SuperScript® III RT is active up to 55°C, making it ideal for use with gene-specific primers with a high Tm. At this temperature it delivers increased specificity, high yield, and low background with various template sizes, something that other RTs with lower thermostability just can’t do. The high thermostability of SuperScript® III RT also means the secondary structure associated with GC-rich RNA templates is not a problem.

Everything You Need to Succeed

The SuperScript® III First-Strand Synthesis System conveniently provides all the necessary components for optimal first-strand cDNA synthesis. With everything ready to go, you can start generating high-quality first-strand cDNA from total or poly(A)+RNA right away. Used with PCR, this functionally tested system enables you to detect rare messages and generate enough material for cloning.

SuperScript® III First-Strand Synthesis SuperMix

This supermix contains reaction buffer, MgCl2, dNTPS, and random hexamers and oligo(dT) primers in separate tubes to suit any priming strategy.

SuperScript® III First-Strand Synthesis System for RT-PCR

This system contains all the necessary components in separate tubes to allow full optimization of your RT reactions.

Change Location Change Location
  • Youtube
  • Twitter
  • Facebook