One-Step RT-PCR

SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. The high thermostability of SuperScript® III RT with the specificity of Platinum® Taq DNA Polymerase increases priming specificity, increased product yields and identifies a larger range of targets.

  • Contains SuperScript® III RT for higher cDNA yields, and increased thermostability
  • Routine detection down to 0.01 pg total RNA
  • One-step format for speed, convenience, and less reaction-to-reaction variability
  • Order 1-Step RT-PCR Kits

Better at 55°C

SuperScript III® RT is included to generate high yields of quality cDNA. SuperScript® III RT is active up to 55°C, making it ideal for use with gene-specific primers with a high melting temperature. At this temperature it delivers increased specificity, high yield, and low background with various template sizes, something that other RTs with lower thermostability just can’t do. The high thermostability of SuperScript® III RT also means the secondary structure associated with GC-rich RNA templates is not a problem.

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