SuperScript® III Reverse Transcriptase

SuperScript® III Reverse Transcriptase (RT) provides higher yields of full-length cDNA for more complete gene product representation compared to other RT enzymes. Robust cDNA synthesis is a critical component of gene expression research. Suboptimal RT reactions can lead to low yields of cDNA that compromise sensitivity or truncated cDNA that can lead to false negative results. Most reverse transcription enzymes fail to consistently produce cDNA with full representation of input RNA due to significant RNase H activity and limited thermostability. SuperScript® III RT has been engineered for higher thermostability and a longer half-life at 50°C. In addition SuperScript® III exhibits reduced RNase H activity. If you need maximum sensitivity for real time RT-PCR and microarray experiments and cDNA that represents input RNA with maximum fidelity, you need SuperScript® III RT.

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For a limited time buy 2 & get 1 FREE! - Save on SuperScript® Reverse Transcriptases

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Products Containing SuperScript® III Reverse Transcriptase

cDNA Synthesis direct from cells
SuperScript® III CellsDirect cDNA Synthesis Kits deliver high yields of first-strand cDNA directly from cells, without the need for costly, time consuming RNA purification procedures.

  

First-Strand cDNA Synthesis
SuperScript® III First-Strand Synthesis Kits are available in multiple formats containing SuperScript® III RT, RNaseOUT™, dNTPs, randon hexamers and oligo(dt)20

  

End-point cDNA Synthesis
SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase provide the highest sensitivity and specificty for one-step RT-PCR for end point detection

SuperScript™ III Compared to SuperScript™ II & M-MLV RT's

An autoradiograph is shown of 32P-labeled cDNA synthesized from a mixture of 0.25 µg of RNA each of 1.35 kb, 2.4 kb, 4.4 kb, 7.5 kb and 9.5 kb with 200 units of each RT at various temperatures. Lane M is 32P-labeled 1 kb DNA ladder.

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