Reverse Transcription for Real-Time PCR

Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of cDNA to improve the dynamic range of your real time RT-PCR assays. Life Technologies offers a wide range of Reverse Transcriptase enzymes optimized for generating cDNA for real- time PCR. With brands such as Applied Biosystems’ High Capacity cDNA Reverse Transcriptase Kit and Invitrogen’s Superscript VILO, Life Technologies has what you need for successful cDNA generation to meet your research needs.

Which reverse transcription enzyme is right for you?

  Standard real-time cDNA synthesis Standard Easy to Use real-time cDNA synthesis For high temperature cDNA synthesis High performance real-time cDNA synthesis High performance Easy to Use real-time cDNA synthesis
Reverse Transcriptase MultiScribe MultiScribe ThermoScript™ Superscript III Superscript III
Top Seller       yes  
Optimal Reaction Temp. 37°C 37°C 65°C 42°C 42°C
Price/Rxn $0.96
(200 Rxn) 		$3.72
(50Rxn)		 $5.23
(100 Rxn)		 $7.72
(250 Rxn) 		$7.81
(250 Rxn)
Sensitivity 20ng-2ug Total RNA 20ng-2ug Total RNA 1pg-1ug Total RNA 1pg-2.5ug Total RNA 1pg-2.5ug Total RNA
Reduced RNase H Activity     yes
(moderate)		 yes
(high)		 yes
(high)
Kit Format Five-tube kit Two-Tube Two-Tube Two-Tube Single tube Mastermix
Designed for real-time PCR yes yes yes yes yes
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