Reverse Transcription for Cloning
Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of quality full length cDNA that accurately represents the input RNA. Life Technologies offers a wide range of Reverse Transcriptase enzymes optimized for generating cDNA for your research needs. Choose from a variety of enzyme types and formats, including SuperScript® III, SuperScript® II and MMLV. Life Technologies has what you need for successful cDNA generation.
Which Reverse Transcription Enzyme Is Right for You?
|General-Purpose cDNA Synthesis||High Performance cDNA Synthesis||Standard High Temperature cDNA||For High Temperature cDNA Synthesis|
|Optimal Reaction Temp.||42°C||50°C||55°C||65°C|
(for largest size)
|Max cDNA Length||Up to 12.3 kb||Up to 12.3 kb||Up to 12.3 kb||Up to 12.3 kb|
|Sensitivity||10 pg - 5 µg Total RNA||10 pg - 5 µg Total RNA||10 pg - 5 µg Total RNA||10 pg - 5 µg Total RNA|
|Reduced RNase H Activity||(high)||(high)||(moderate)|
|RT-PCR Kits Format|
|Order Now||Order Now||Order Now||Order Now|