Reverse Transcription for Cloning

Choosing the correct reverse transcriptase (RT) for cDNA synthesis is critical to obtaining high yields of quality full length cDNA that accurately represents the input RNA.  Life Technologies offers a wide range of Reverse Transcriptase enzymes optimized for generating cDNA for your research needs. Choose from a variety of enzyme types and formats, including SuperScript® III, SuperScript® II and MMLV. Life Technologies has what you need for successful cDNA generation.

Which Reverse Transcription Enzyme Is Right for You?

  General-Purpose cDNA Synthesis High Performance cDNA Synthesis Standard High Temperature cDNA For High Temperature cDNA Synthesis
Top Seller   yes  
Optimal Reaction Temp. 42°C 50°C 55°C 65°C
Price/Reaction
(for largest size) 		$4.87
(200 reactions) 		$5.03
(200 reactions) 		$5.47
(50 reactions) 		$6.65
(100 reactions)
Max cDNA Length Up to 12.3 kb Up to 12.3 kb Up to 12.3 kb Up to 12.3 kb
Sensitivity 10 pg - 5 µg Total RNA 10 pg - 5 µg Total RNA 10 pg - 5 µg Total RNA 10 pg - 5 µg Total RNA
Reduced RNase H Activity yes (high) yes (high) yes (moderate)
RT-PCR Kits Format yes yes yes
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