Reverse Transcription

Choosing the correct reverse transcriptase for reverse transcription and cDNA synthesis is critical to obtaining high yields of quality full length cDNA with full gene representation. A reverse transcriptase with high temperature performance is important when transcribing RNA with secondary structure or when working with gene-specific primers. Reverse transcription with reduced RNase H activity reverse transcriptase enzymes produces more full length cDNA and greater gene representation.

The SuperScript® III Reverse Transcriptase is the most widely used brand of enzyme for cDNA synthesis, due to the proven ability to deliver reliable and consistently high yields of fully representative full length cDNA.