SYBR® Green Real-Time PCR Master MixesSYBR® Green Master Mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR® Green dye; everything needed for real-time PCR, except the sample and PCR primer pair. |
Which SYBR® Green Master Mix Is Right for You?
| For Reliable Performance | For Fast Performance | For Best Performance | |||
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| Top Seller |  |
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| Cycling Mode | Standard (90 minutes) |
Fast (35 minutes) |
Standard (90 minutes) or Fast mode (30 minutes) |
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| SYBR GreenER™ dye for brighter signal | |
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| Proprietary hot-start mechanism for exceptional specificity | |
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| UDG for Carryover Contamination Control | |
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| Heat-labile UDG allows product to be stable 72 hrs pre and post qPCR | |
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| Sensitivity | 2 copies | 1 copy | |||
| Applications | Gene Expression DNA quantitation (pathogen detection) CHiP |
Gene Expression DNA quantitation (pathogen detection) |
Gene Expression DNA quantitation (pathogen detection) |
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| Instrument Considerations | Not recommended for 7900HT Real-time PCR instruments | ||||
| Primer Considerations | Only to be used with primers with Tm ≥ 60° | Only to be used with primer concentrations <200 nM for StepOne/StepOnePlus Instruments | |||
| Price / 20 µL Reaction | $0.60-$0.98 | $0.68-$1.07 | $0.54-$0.73 |
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Regulatory Status: For Research Use Only. Not for human or animal therapeutic or diagnostic use.
