SYBR® Green Real-Time PCR Master MixesSYBR® Green Master Mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR® Green dye; everything needed for real-time PCR, except the sample and PCR primer pair. |
Which SYBR® Green Master Mix Is Right for You?
| Universal SYBR® Green Master Mix for Standard Instruments | SYBR® Green Master Mix for Fast Cycling Instruments | ||
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| Top Seller |  |
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| Cycling Mode | Standard 90 minute run time |
Fast 35 minute run time |
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| Primer Tm | No special primer Tm requirments | Only to be used with primers with Tm ≥ 60°C | |
| dUTP to Reduce Carryover Contamination | |
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| UDG for Carryover Contamination Control | |
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| ROX Reference Dye to Normalise Signal & Ensure Data Integrity | |
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| Sensitivity | 2 copies | 2 copies | |
| Price / 20 µL Reaction | $0.61-$0.96 | $0.68-$1.07 | |
| Order Now | Order Now |
Other SYBR® Green Real-Time Master Mixes
The following mixes contain heat-labile UNG, and also provide increased brightness.
EXPRESS SYBR® GreenER™ qPCR Supermix with Premixed ROX | |
| Superior real-time master mix with UDG contamination control for sensitivity and reproducibility using fast-cycling instruments. | Order Now |
EXPRESS SYBR® GreenER™ qPCR Supermix Universal | |
| High performing real-time master mix with superior sensitivity and reproducibility using standard-cycling instruments. | Order Now |
