SYBR® Green Real-Time PCR Master Mixes

SYBR® Green Master Mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes contain buffer, dNTPs, thermostable hot-start DNA polymerase, and of course SYBR® Green dye; everything needed for real-time PCR, except the sample and PCR primer pair.

SYBR® Green dye intercalates into double-stranded DNA (dsDNA), creating more fluorescent signal as PCR product accumulates. Since only two PCR primers are needed for each target, it may be easier to design primers comapred to the TaqMan® Assay-based detection method. However, because SYBR® Green dye will bind to any dsDNA molecule, poor primer design can result in amplification signal from non-specific and non-target PCR products. We recommend always checking reaction products by low resolution melt analysis after qPCR using SYBR® Green dye–based detection to verify that the primary product consists of a single amplicon.

Fast SYBR® Green Master Mix

A fast, reliable, and cost-effective solution for your real-time PCR applications that helps deliver sensitivity, specificity, dynamic range, and PCR efficiency. Compatible with Fast cycling protocols and systems.

Power SYBR® Green Master Mix

Power SYBR® Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene and providing linear results from a broad range of input-template amounts.

Formulated for Great Results on Our Systems and Yours

Instruments & Master MixesRoche LC480Bio-Rad iQ5Bio-Rad CFX96
    
Fast SYBR® Green
Master Mix
Power SYBR® Green
PCR Master Mix   

SYBR® Green Real-Time Master Mixes

Fast SYBR® Green Master Mix

  • Superior real-time master mix with UDG contamination control for sensitivity and reproducibility using fast-cycling instruments

Power SYBR® Green PCR Master Mix

  • High performing real-time master mix with superior sensitivity and reproducibility using standard-cycling instruments

EXPRESS SYBR® GreenER™ qPCR Supermix with Premixed ROX

  • Real-time PCR master mix with SYBR Greener and ROX reference dye designed for fast and standard cycling instruments

EXPRESS SYBR® GreenER™ qPCR Supermix Universal

  • Real-time PCR master mix with SYBR Greener and a separate tube of ROX reference dye designed for fast and standard cycling instruments

SYBR® Green PCR Master Mix

  • Convenient master mix for routine real-time PCR analysis

Platinum® SYBR® Green qPCR SuperMix-UDG with Premixed ROX

  • Real-time PCR master mix with ROX reference dye and UDG contamination control

Platinum® SYBR® Green qPCR SuperMix-UDG

  • Real-time PCR master mix with UDG contamination control and a separate tube of ROX reference dye

SYBR GreenER™ qPCR SuperMix for ABI PRISM® instruments

  • Real-time PCR master mix with SYBR Greener and ROX reference dye designed for ABI PRISM instruments

SYBR GreenER™ qPCR SuperMix Universal

  • Real-time PCR master mix with SYBR Greener and a separate tube of ROX reference dye designed for standard-cycling instruments

SYBR GreenER™ qPCR SuperMix for iCycler® instruments

  • Real-time PCR master mix with SYBR Greener and fluorescein for iCycler iQ® or MyiQ® instruments
See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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