EXPRESS One-Step SuperScript® qRT-PCR Kits
EXPRESS One-Step SuperScript® qRT-PCR SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, Fast-cycling instruments for your probe-based qPCR experiments.
These kits provide:
One-Step Reactions and Rapid Cycling
EXPRESS One-Step SuperScript® qRT-PCR Kits provide components for one-step reverse transcription (RT) and quantitative PCR (qRT-PCR) in a convenient format that is compatible with both rapid and standard qPCR cycling conditions. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers and either total RNA or mRNA. These one-step qRT-PCR kits have been formulated for use with TaqMan® probe-based technology.
High Performance Enzymes and Reagents Formulated for Convenience
The RT mix includes SuperScript® III Reverse Transcriptase and RNaseOUT™ Recombinant Ribonuclease Inhibitor. The EXPRESS qPCR SuperMix includes Platinum® Taq DNA polymerase, MgCl2, dNTPs (with dUTP instead of dTTP), heat-labile uracil DNA glycosylase (UDG), and stabilizers. Note that this unique one-step formulation includes a special form of UDG in the SuperMix to help prevent re-amplification of carryover PCR products between reactions.
- SuperMix with Premixed ROX™ dye: The qPCR SuperMix with premixed ROX™ dye includes ROX™ dye Reference Dye at a final concentration of 500 nM to normalize the fluorescent signal on instruments that are compatible with this option, including most Applied Biosystems® real-time PCR instruments
- Universal SuperMix: The Universal SuperMix includes ROX™ dye as a separate component for instruments that use ROX™ at a different concentration or do not require ROX™ dye. Examples of instruments that use ROX™ dye at a lower concentration are the Applied Biosystems® 7500 and ViiA™ 7 System