One-Step Real-Time RT-PCR Master MixesOne-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments. |
Which One-Step Real-Time RT-PCR Master Mix Is Right for You?
| Excellent Results for Everyday Applications | Enabled for Fast Cycling When Time Counts | Highest Overall Performance ( Sensitivity, Speed, and Tolerance to Inhibitors) | |||
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| Detection Chemistry | TaqMan® SYBR® |
TaqMan® SYBR® |
TaqMan® | ||
| Sensitivity | High | High | High | ||
| Cycling/Run Time | 120 minutes | <60 min | <60 min | ||
| Tolerant to PCR Inhibitors |  |
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| Applications | Gene expression RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Multiplexing |
Gene expression RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Multiplexing |
Gene expression RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Multiplexing |
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| UDG to Prevent DNA Carryover Contamination | |
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| Reverse Transcriptase | Array Script UP | SuperScript III | Thermostable MMLV | ||
| ROX Reference Dye to Normalize Signal & Ensure Data Integrity | |
versions with and without | |
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| Price per 20ul rxn | $588 for 500 rxns | $711 for 500 rxns | $1442 for 1000 rxns | ||
| Format | 2 Tubes (20X RT Mix, 2X qPCR Mix) | 2 Tubes (10X RT Mix, 2X qPCR Mix) | 1 Tube (4X Mix) |
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