One-Step Real-Time RT-PCR Master Mixes
One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments. |
EXPRESS One-Step SuperScript® qRT-PCR Kits
For gene expression studies in a one-step format using TaqMan® Assays or other primer-probe qPCR chemistry. Use it with our comprehensive library of predesigned TaqMan® Assays for gene expression to quickly start generating reliable results. The kits can be used for Fast or standard cycling protocols, and include PCR carryover control reagents (UDG). These kits are an update to the popular SuperScript® 3 Platinum® One-Step qRT-PCR Kits. Available with or without ROX™ dye.
General-Purpose 1-Step Real-Time RT-PCR Kits
TaqMan® RNA-to-CT™ 1-Step Kits and Power SYBR® Green RNA-to-CT 1-Step Kits
General-purpose one-step real-time RT-PCR kits at an affordable price. Available for TaqMan® Assay or SYBR® Green Dye chemistry, with premixed ROX™ passive reference dye. The TaqMan® RNA-to-CT™ 1-Step Kit is designed to take advantage of the comprehensive library of predesigned TaqMan® Gene Expression Assays.
TaqMan® Fast Virus 1-Step Master Mix
A sensitive, robust master mix optimized for virus detection and compatible with Fast or standard cycling protocols. Enhances quantification of viral RNA and DNA even with samples that contain common RT-PCR inhibitors.
