Real-Time PCR Reagents & Kits
Real-time PCR is a versatile technique that is used in many different research applications. And, for research applications such as gene expression analysis, there are many ways to conduct your experiments. Find optimized real-time PCR master mixes, reagents, and kits to power your experiments here. |
TaqMan® Master MixesTaqMan® Master Mixes contain the buffers, dNTPs, internal reference dye, and - most importantly - hot-start DNA polymerase, that power real-time PCR. They were developed for use with our comprehensive selection of TaqMan® Assays to streamline reaction setup and therefore minimize variability among replicates, assays, and experiments. |
SYBR® Green Master MixesIn SYBR® Green-based real-time PCR, amplification products are detected with fluorescent signal released by SYBR® Green dye, which fluoresces only when bound to double-stranded DNA. Our SYBR® Green Master mixes include buffers, dNTPs, internal reference dye, hot-start DNA Polymerase, and of course, SYBR® Green dye. |
Reverse Transcription for Real-Time PCR
RNA must be converted to cDNA in order to be amplified using PCR. We recommend the Invitrogen® SuperScript® VILO™ cDNA Synthesis Kit for reverse transcription prior to analyzing gene expression (mRNA), long noncoding RNA, and pri-miRNA using real-time PCR. For analysis of microRNA and other small RNAs using TaqMan® Assays, we recommend the TaqMan® MicroRNA Reverse Transcription Kit.
One-Step Real-Time RT-PCR Kits
In one-step RT-PCR, reagents and enzymes for both reverse transcription (RT) and real-time PCR are added to RNA samples at the same time. Reactions are first incubated using conditions favorable for RT, then the resulting cDNA is immediately run in a real-time PCR reaction.
Real-Time PCR Directly from Samples
With Sample-to-Answer Kits, crude sample lysates can be used directly in real-time PCR or one-step RT-PCR. No nucleic acid purification is required, saving time and reducing the chance of sample mixup or cross-contamination. We recommend the Ambion® Cells-to-CT™ Kits for gene expression and miRNA studies. For SNP genotyping we recommend the TaqMan® Sample-to-SNP™ Kit.
High Resolution Melt (HRM) Master Mix
Mixed with your DNA sample and PCR primers, MeltDoctor™ HRM Master Mix provides the reagents needed to discriminate subtle differences in the target DNA sequence using post-PCR melting analysis.
TaqMan® OpenArray® Reagents
Specialized master mixes for use on the OpenArray® system.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Kits and controls for establishing and maintaining your system’s interpretation of fluorescence data collected during real-time PCR. We recommend that labs run calibration and verification experiments at least every six months for optimal results.
- See all our Real-Time PCR Instrument Calibration Kits
